Dual Characteristics of Apoptosis and Survival in Cerebellar Purkinje Cells Four Days After Transient Global Ischemia.

Zhen He, Alena Savenka, Ada G Cino Ozuna, Kelly J Davis, Angel Paredes

Abstract read

In one paragraph

Article in Cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Zhen HeDivision of Neurotoxicology, HFT-132, National Center for Toxicological Research, Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA.ORCID 0000-0001-9972-9114

Alena SavenkaNanotechnology Core Facility, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA.

Ada G Cino OzunaToxicologic Pathology Associates, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA.

Kelly J DavisToxicologic Pathology Associates, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA.

Angel ParedesNanotechnology Core Facility, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Whether apoptotic cell death occurs in cerebellar Purkinje cells following transient global ischemia remains unclear. Histologic, immunofluorescent and ultramicroscopic methods were used to assess ischemic outcomes in rats. A pilot study using fluorescence labeling [TUNEL-caspase 3-activated peptide (C3AP)-DAPI] revealed key apoptotic characteristics including nuclear TUNEL-positive, nuclear membrane blebs (NMBs), and cytoplasmic C3AP-positive structures, in addition to transport of TUNEL-positive round structures into cytoplasm and projections in ischemic Purkinje cells but not in controls 4 days after ischemia. A formal follow-up study confirmed that 8% of Purkinje cells 4 days following ischemia exhibited TUNEL-positivity and/or NMBs, while Purkinje cells in controls did not. TUNEL-positive Purkinje cells displayed reduced intensity of Calbindin D28K-ifl/MitoTracker (living cell markers) labeling as compared to controls (

Indexed as

ApoptosisBrain IschemiaCerebellumPurkinje CellsAnimalsCell SurvivalFemaleMaleRatsRats, Inbred F344Rats, Sprague-Dawleycerebellar Purkinje cellsglobal ischemiamitochondria fragmentation and lossnon-canonical apoptosisnuclear apoptotic bodynuclear membrane blebbingTUNEL-positive

Identifiers

PMID41972663

DOI10.3390/cells15070572

PMCPMC13072376

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.