Evidence map›Paper›PMID 41972538›Full record

ArticleBiology2026

CRISPR/Cas9-Mediated Polyketide Synthase Replacement for High-Yield Biosynthesis and Biological Activity of Milbemycin D.

Shenchen Tao, Huan Qi, Xian Luo, Jingyi Shen, Yunfei He, Jun Huang, Ruijun Wang, Shaoyong Zhang, Yongsheng Gao, Jidong Wang and 1 more

Abstract read
In one paragraph

Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shenchen TaoCollaborative Innovation Center of Green Pesticide, School of Forestry and Biotechnology, Zhejiang A & F University, No. 666 Wusu Street, Linan District, Hangzhou 311300, China.ORCID 0009-0006-5136-0120
Huan QiZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.
Xian LuoZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.
Jingyi ShenZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.
Yunfei HeZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.ORCID 0009-0006-4966-3907
Jun HuangZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.
Ruijun WangZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.
Shaoyong ZhangZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.
Yongsheng GaoZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.
Jidong WangZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College Life Science, Huzhou University, No. 759 Erhuandong Road, Wuxing District, Huzhou 313000, China.
Liqin ZhangCollaborative Innovation Center of Green Pesticide, School of Forestry and Biotechnology, Zhejiang A & F University, No. 666 Wusu Street, Linan District, Hangzhou 311300, China.

Funding

the National Key Research and Development Program of China 2021YFC2600104
6 · The paper itself

Abstract

Milbemycin D is a promising 16-membered macrolide insecticide with reported superior efficacy, but its commercial development has been hindered by extremely low natural yields. This study aimed to construct a high-yielding microbial platform for milbemycin D production using combinatorial biosynthesis and advanced genome editing. An optimized CRISPR/Cas9-AcrIIA4 system was employed to seamlessly replace the

Indexed as

avermectinsbiological activitycombinatorial biosynthesisCRISPR/Cas9genome editingmilbemycinspolyketide synthase

Identifiers

PMID41972538
PMCPMC13071997

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.