ArticleBiochemia medica2026
Effects of different blood storage conditions on leukocytes cell population data using the Sysmex XN hematological analyzer.
Article in Biochemia medica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: The aim of this study was to investigate the stability of leukocyte cell population data (CPD) assessed on Sysmex XN-3000 hematological analyzer in samples stored for up to 24 h at different temperatures. Materials and methods: Whole-blood samples from 25 donors were analyzed for 18 CPD parameters at baseline and after storage at room temperature (RT), 4 °C, or 37 °C for 3, 6, 12, and 24 h. Stability was assessed using three complementary approaches: paired Wilcoxon tests with Bonferroni correction, percent difference between medians > 10%, and Results: According to predefined stability criteria, several CPD parameters exceeded stability limits within 24 h, particularly under storage at 37 °C. Parameters related to neutrophil, lymphocyte and monocyte complexity and size ( Conclusions: Leukocyte CPD stability is strongly temperature- and parameter-dependent. Exposure to elevated temperature (37 °C) leads to early exceedance of predefined stability limits for several CPD parameters, whereas storage at RT preserves stability for longer time intervals. Avoiding prolonged exposure to high temperatures is therefore essential for reliable CPD assessment in routine laboratory practice.
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