Individually addressable multichannel nanoelectrodes reveal spatially resolved functional heterogeneity of vesicles in single cells.

Chuchu Xu, Ruolin Liu, Yuhao Zheng, Yue Chen, Irina Svir, Alexander Oleinick, Zhongqun Tian, Christian Amatore, Keke Hu

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In one paragraph

Article in Chemical science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Chuchu XuState Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China.

Ruolin LiuState Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China.

Yuhao ZhengState Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China.

Yue ChenState Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China.

Irina SvirChimie Physique et Chimie du Vivant, Département de Chimie, École Normale Supérieure PSL University, Sorbonne Université, CNRS 24 rue Lhomond 75005 Paris France.ORCID https://orcid.org/0000-0002-6006-3148

Alexander OleinickChimie Physique et Chimie du Vivant, Département de Chimie, École Normale Supérieure PSL University, Sorbonne Université, CNRS 24 rue Lhomond 75005 Paris France.ORCID https://orcid.org/0000-0001-6194-0459

Zhongqun TianState Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China.ORCID https://orcid.org/0000-0002-9775-8189

Christian AmatoreState Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China.ORCID https://orcid.org/0000-0002-2561-1137

Keke HuState Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China.ORCID https://orcid.org/0000-0002-6663-6832

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nanoelectrodes are powerful tools for high-resolution electrochemical measurements, yet a long-standing limitation has been the inability to perform simultaneous, spatially resolved measurements across multiple domains within a single confined system. Here we develop an individually addressable multichannel nanoelectrode platform that preserves nanoscale spatial resolution while enabling independent and parallel electrochemical recording. The platform supports batch fabrication of nanoelectrodes with either hollow or filled architectures, providing precise geometric control for applications ranging from confined intracellular environments to open electrochemical interfaces, as validated by electron microscopy, voltammetry, and simulations. Applied to single living cells, hollow multichannel electrodes enable minimally disruptive, region-resolved intracellular recording from nuclear-adjacent and membrane-proximal domains, providing direct quantitative evidence for functional heterogeneity of vesicle populations. These findings uncover a previously inaccessible mechanistic link between subcellular vesicle organization and secretion efficiency, with direct implications for how neuroendocrine and neuronal cells achieve rapid yet regulated chemical signaling.

Identifiers

PMID41971236

DOI10.1039/d6sc01178k

PMCPMC13066938

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.