A Chimeric LBT-GFP Biosensor Exhibits Antithetical Fluorescence Responses to Ca

Jingyao Li, Sandra Oloketuyi, Li-Wei Hung, Nilusha Sudasinghe, Sang-Min Shin, Ramesh K Jha

Abstract read

In one paragraph

Article in ACS omega, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jingyao LiBioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.ORCID https://orcid.org/0000-0002-6834-4681

Sandra OloketuyiBioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.

Li-Wei HungBioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.ORCID https://orcid.org/0000-0001-6690-8458

Nilusha SudasingheChemistry Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.

Sang-Min ShinBioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.ORCID https://orcid.org/0000-0002-5794-5510

Ramesh K JhaBioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States.ORCID https://orcid.org/0000-0001-5904-3441

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rare earth elements (REEs) are critical components in emerging technologies, but their mining and refining processes are often laborious, costly, and environmentally damaging. Developing green and efficient separation methods for REEs is crucial. Biomolecular approaches using lanthanide-binding proteins and peptides show promise for selective REE extraction and separation. In this study, we present the design and characterization of a genetically encoded fluorescence indicator (GEFI) construct that combines a superfolder green fluorescent protein (sfGFP) with a dual lanthanide-binding tag (2×dLBT). The 2×dLBT insert induces conformational changes in sfGFP upon lanthanide binding, modulating the fluorescence intensity. The sfGFP-2×dLBT biosensor exhibited distinct fluorescence responses to different lanthanide ions, with the highest dynamic range observed for heavy REEs like dysprosium (Dy

Identifiers

PMID41970837

DOI10.1021/acsomega.5c10135

PMCPMC13063197

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.