Evidence map›Paper›PMID 41969984›Full record

ArticleSleep and biological rhythms2026

Mitophagy protects renal tubular epithelial cells from intermittent hypoxia-induced injury via the HIF-1α/BNIP3 pathway.

Xiao-Bin Zhang, Qi-Feng Gan, Yu-Xin Guo, Li-Da Chen, Li-Qin Zhang, Ling Cai, Ya-Ting Yuan, Jing-Huang Cai, Xiang-Xin Chen

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Article in Sleep and biological rhythms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Xiao-Bin Zhang *Department of Pulmonary and Critical Care Medicine, School of Medicine, Zhongshan Hospital of Xiamen University, Xiamen University, No. 201, Hubin South Road, Siming District, Xiamen, 361004 Fujian Province People's Republic of China.
Qi-Feng Gan *School of Medicine, Xiamen University, Xiamen, Fujian People's Republic of China.
Yu-Xin Guo *School of Medicine, Xiamen University, Xiamen, Fujian People's Republic of China.
Li-Da Chen *Department of Pulmonary and Critical Care Medicine, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian People's Republic of China.
Li-Qin Zhang *Department of Oncology, Medicine Center of Xiamen University, Xiang'an Hospital of Xiamen University, Xiamen, Fujian People's Republic of China.
Ling Cai *Department of Pulmonary and Critical Care Medicine, School of Medicine, Zhongshan Hospital of Xiamen University, Xiamen University, No. 201, Hubin South Road, Siming District, Xiamen, 361004 Fujian Province People's Republic of China.
Ya-Ting Yuan *Department of Pulmonary and Critical Care Medicine, School of Medicine, Zhongshan Hospital of Xiamen University, Xiamen University, No. 201, Hubin South Road, Siming District, Xiamen, 361004 Fujian Province People's Republic of China.
Jing-Huang Cai *Department of Pulmonary and Critical Care Medicine, School of Medicine, Zhongshan Hospital of Xiamen University, Xiamen University, No. 201, Hubin South Road, Siming District, Xiamen, 361004 Fujian Province People's Republic of China.
Xiang-Xin Chen *Department of Pulmonary and Critical Care Medicine, School of Medicine, Zhongshan Hospital of Xiamen University, Xiamen University, No. 201, Hubin South Road, Siming District, Xiamen, 361004 Fujian Province People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to investigate the role of mitophagy mediated by the hypoxia-inducible factor-1α (HIF-1α)/Bcl-2/adenovirus E1B 19-kDa interacting protein (BNIP3) pathway in mitigating renal injury induced by intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA). Human renal tubular epithelial cells (RTECs) were exposed to IH conditions using a hypoxia-reoxygenation chamber for 24 h. Cells were divided into five groups: normoxia, IH, IH with HIF-1α siRNA (IH + si-HIF-1α), IH with BNIP3 siRNA (IH + siBNIP3), and IH with HIF-1α siRNA plus BNIP3 overexpression (IH + si-HIF-1α + BNIP3). Cell viability, apoptosis, mitochondrial morphology, and mitophagy levels were assessed using flow cytometry, western blotting, transmission electron microscopy, and immunofluorescence. Under IH conditions, inhibition of HIF-1α or BNIP3 significantly reduced cell viability, increased apoptosis, disrupted mitochondrial structure, and decreased mitophagy levels in RTECs. Overexpression of BNIP3 in the presence of HIF-1α inhibition restored mitophagy levels, attenuated cellular damage and apoptosis, and improved mitochondrial morphology. These findings demonstrate that mitophagy mediated by the HIF-1α/BNIP3 signaling pathway plays a protective role in IH-induced renal injury, suggesting that targeted enhancement of mitophagy may provide a potential therapeutic strategy for OSA-related kidney dysfunction. Supplementary Information: The online version contains supplementary material available at 10.1007/s41105-025-00625-5.

Indexed as

BNIP3HIF-1αIntermittent hypoxiaMitophagyRenal tubular epithelial cells

Identifiers

PMID41969984
PMCPMC13066096

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