ArticleSe pu = Chinese journal of chromatography2026
[Determination of ergot alkaloids toxins in forage grass by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid-phase supported liquid-liquid extraction].
Article in Se pu = Chinese journal of chromatography, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Toxic alkaloids， especially ergot alkaloid toxins， present in forage grass and pose serious health hazards to humans and livestock. Hence， a method for simultaneously detecting these dangerous plant toxins is needed. This study established a rapid method for detecting ten ergot alkaloid toxins using solid-phase supported liquid-liquid extraction technology combined with ultra performance liquid chromatography-tandem mass spectrometry. Forage samples were extracted with 8.0 mL of acetonitrile containing 1% formic acid. The extraction mixture was vortexed and then centrifuged at 8 000 r/min for 5 min， after which 1.0 mL of the supernatant was mixed with 3.0 mL of water and vortexed for 0.5 min. The entire mixture was transferred to a solid-phase supported liquid-liquid extraction column and allowed to stand for 10 min. The column was eluted with 6.0 mL of ethyl acetate and the collected eluate was evaporated under nitrogen at temperatures below 40 ℃. The residue was dissolved in 1 mL of acetonitrile-water （1∶3， volume ratio， containing 0.5% formic acid）， and separated using an Acquity UPLC BEH C
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