Evidence map›Paper›PMID 41968968›Full record

ArticleTransfusion2026

An in-house nucleic acid test for detection of Anaplasma phagocytophilum in whole blood donor samples.

Dilini Kumaran, Carmen Charlton, Chantale Pambrun, Steven J Drews, Sandra Ramirez-Arcos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transfusion, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dilini KumaranSurveillance and Discovery Laboratory, Canadian Blood Services, Ottawa, Ontario, Canada.
Carmen CharltonSurveillance and Discovery Laboratory, Canadian Blood Services, Ottawa, Ontario, Canada.ORCID https://orcid.org/0000-0002-9129-5136
Chantale PambrunInnovation & Portfolio Management, Canadian Blood Services, Ottawa, Ontario, Canada.
Steven J DrewsSurveillance and Discovery Laboratory, Canadian Blood Services, Ottawa, Ontario, Canada.ORCID https://orcid.org/0000-0003-2519-1109
Sandra Ramirez-ArcosSurveillance and Discovery Laboratory, Canadian Blood Services, Ottawa, Ontario, Canada.ORCID https://orcid.org/0000-0003-0705-4987

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAnaplasma phagocytophilum is a tick-borne pathogen that causes anaplasmosis. Increased incidences of this disease in Canada and cases of transfusion-transmitted anaplasmosis in the United States have been reported. Currently, there are no Health Canada licensed methodologies available for detection of A. phagocytophilum nucleic acids (NAs) in blood donors. Here we describe a method for NA extraction and detection of A. phagocytophilum in whole blood (WB) donor samples. STUDY DESIGN AND

methodsPlasmids carrying the A. phagocytophilum msp2 and gltA genes and genomic A. phagocytophilum DNA were used to spike WB (10

resultsA. phagocytophilum msp2 and gltA were detected by qPCR in plasmid-spiked WB at a concentration of 10

conclusionA simple, scalable in-house protocol for NA extraction from RWBCT has been optimized to detect A. phagocytophilum in WB donor samples.

Indexed as

Anaplasma phagocytophilumBlood DonorsDNA, BacterialHumansDNA, Bacterial

Identifiers

PMID41968968
PMCPMC13250363

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.