Evidence map›Paper›PMID 41968248›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Development and Validation of a Real-Time PCR Assay for the Detection of Aspergillus fumigatus on Panther Fusion System.

Margherita Cacaci, Damiano Squitieri, Maurizio Sanguinetti, Brunella Posteraro

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Margherita CacaciDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
Damiano SquitieriDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
Maurizio SanguinettiDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy. maurizio.sanguinetti@unicatt.it.
Brunella PosteraroDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This protocol aims to provide a detailed description of the development and clinical validation of a laboratory-developed test (LDT) for the detection of Aspergillus fumigatus in respiratory specimens using the Panther Fusion Open Access system. This platform allows automated extraction of the pathogen genomic DNA and amplification of targets. Specifically, the assay targets the ITS1 region of fungal ribosomal DNA through a real-time PCR workflow. Specificity of primers and probe was verified in silico, and assay parameters, such as MgCl

Indexed as

Aspergillus fumigatusReal-Time Polymerase Chain ReactionDNA, FungalHumansLimit of DetectionSensitivity and SpecificityDNA, FungalInvasive-fungal infectionLaboratory-developed test (LDT)Molecular diagnosisPanther FusionPulmonary aspergillosisPulmonary infectionReal-time PCR

Identifiers

PMID41968248

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.