Evidence map›Paper›PMID 41966829›Full record

ArticleSTAR protocols2026

Protocol for the assessment of the impact of mycotoxins and glyphosate residues on the gut microbiome and resistome of European fallow deer.

Adrienn Gréta Tóth, Melinda Paholcsek, Norbert Solymosi, Anikó Stágel, Patrik Gömbös, Katalin Posta, István Lakatos, Sára Ágnes Nagy, Szilamér Ferenczi, Zsuzsanna Szőke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Adrienn Gréta TóthInstitute for Animal Breeding, Nutrition and Laboratory Animal Science, University of Veterinary Medicine, 1078 Budapest, Hungary. Electronic address: tothadrienngreta@gmail.com.
Melinda PaholcsekComplex Systems and Microbiome-innovations Centre, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
Norbert SolymosiCentre for Bioinformatics, University of Veterinary Medicine, 1078 Budapest, Hungary; Department of Physics of Complex Systems, Eötvös Loránd University, 1117 Budapest, Hungary.
Anikó StágelHungarian National Blood Transfusion Service Nucleic Acid Testing Laboratory, Budapest, Hungary.
Patrik GömbösAgrobiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Department of Physiology and Animal Health, Hungarian University of Agriculture and Life Sciences, 7400 Kaposvár, Hungary.
Katalin PostaDepartment of Microbiology and Applied Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöllő 2100, Hungary.
István LakatosDepartment of Animal Biotechnology, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöllő 2100, Hungary.
Sára Ágnes NagyDepartment of Physics of Complex Systems, Eötvös Loránd University, 1117 Budapest, Hungary.
Szilamér FerencziDepartment of Microbiology and Applied Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöllő 2100, Hungary; Laboratory of Molecular Neuroendocrinology, Institute of Experimental Medicine, Hungarian Research Network, Budapest, Hungary.
Zsuzsanna SzőkeDepartment of Animal Biotechnology, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöllő 2100, Hungary. Electronic address: ferenczi.szokezsuzsi@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol to describe the bacteriome of the intestinal content of toxin-exposed fallow deer. We describe steps for measuring fecal mycotoxin (deoxynivalenol, zearalenone, fumonisin B1, and aflatoxin B1) levels using liquid chromatography-mass spectrometry, as well as serum glyphosate. We then detail a short-read shotgun DNA sequencing-based bioinformatic pipeline for the toxin level-associated analysis of the bacteriome and resistome and the construction of metagenome-assembled bacterial genomes. This protocol has potential applications in further toxin level-associated metagenome studies. For complete details on the use and execution of this protocol, please refer to Tóth et al.

Indexed as

DeerGastrointestinal MicrobiomeGlycineMycotoxinsAnimalsChromatography, LiquidComputational BiologyFecesGlyphosateMetagenomeShotgun SequencingGlycineGlyphosateMycotoxinsBioinformaticsGenomicsMicrobiologySequencing

Identifiers

PMID41966829
PMCPMC13091997

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.