Evidence map›Paper›PMID 41964677›Full record

ArticleApplied microbiology and biotechnology2026

A novel Azospirillum vistecanum strain from methane digestate promotes plant growth via indole-3-acetic acid biosynthesis.

Surat Moomthong, Thamonwan Woraruthai, Charndanai Tirapanampai, Sasithon Rungjroenchaiwat, Worarat Kruasuwan, Pichahpuk Uthaipaisanwong, Kanthida Kusonmano, Piroon Jenjaroenpun, Thidathip Wongsurawat, Thanyaporn Wongnate

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Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Surat MoomthongSchool of Biomolecular Science & Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
Thamonwan WoraruthaiSchool of Biomolecular Science & Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
Charndanai TirapanampaiSchool of Biomolecular Science & Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
Sasithon RungjroenchaiwatSchool of Biomolecular Science & Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
Worarat KruasuwanDivision of Medical Bioinformatics, Department of Research, Faculty of Medicine Siriraj Hospital, Oxford Nanopore Centre of Excellence, Mahidol University, Bangkok, 10700, Thailand.
Pichahpuk UthaipaisanwongSystems Biology and Bioinformatics Research Group, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
Kanthida KusonmanoSystems Biology and Bioinformatics Research Group, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
Piroon JenjaroenpunDivision of Medical Bioinformatics, Department of Research, Faculty of Medicine Siriraj Hospital, Oxford Nanopore Centre of Excellence, Mahidol University, Bangkok, 10700, Thailand.
Thidathip WongsurawatDivision of Medical Bioinformatics, Department of Research, Faculty of Medicine Siriraj Hospital, Oxford Nanopore Centre of Excellence, Mahidol University, Bangkok, 10700, Thailand.
Thanyaporn WongnateSchool of Biomolecular Science & Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand. thanyaporn.w@vistec.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The urgent need for sustainable agricultural inputs has accelerated the search for microbial alternatives to synthetic agrochemicals. In this study, we report the isolation and comprehensive characterization of a novel strain, Azospirillum vistecanum VT-I1, obtained from a methane-enriched digestate system. This strain demonstrated exceptional plant growth-promoting potential through the biosynthesis of indole-3-acetic acid (IAA), a key phytohormone. Genome sequencing and annotation revealed the presence of genes associated with the indole-3-pyruvate (IPyA) pathway, aro9, ipdC, and aldA, with no detectable IAA-degrading gene clusters, supporting a high net auxin yield. Under optimized culture conditions, VT-I1 produced up to 1.206 mM of IAA, significantly surpassing the levels observed in A. brasilense. Functional assays confirmed the bioactivity of this microbial IAA, which enhanced root development in Exacum affine and improved seed germination in Andrographis paniculata. Collectively, these results establish A. vistecanum VT-I1 as a promising candidate for next-generation biofertilizers, offering a scalable, eco-friendly alternative to chemically synthesized auxins. This work expands our understanding of auxin biosynthesis in rhizobacteria and provides a strong foundation for future field applications and microbiome-based crop enhancement strategies. KEY POINTS: • A novel Azospirillum vistecanum was isolated from methane digestate. • The strain produces high levels of indole-3 acetic acid via the IPyA pathway. • Microbial IAA enhances root growth and seed germination in model plants.

Indexed as

AzospirillumIndoleacetic AcidsMethanePlant DevelopmentPlant Growth RegulatorsIndolesPlant Rootsindol-3-yl pyruvic acidindoleacetic acidIndoleacetic AcidsIndolesMethanePlant Growth RegulatorsGenome-enabled strain characterizationIndole-3-pyruvate pathwayMicrobial biofertilizerRhizosphere-associated bacteriaTryptophan-dependent auxin biosynthesis

Identifiers

PMID41964677
PMCPMC13180765

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.