Evidence map›Paper›PMID 41963787›Full record

ArticleBMC plant biology2026

Unveiling genetic loci and natural alleles for seed iron accumulation in adzuki bean via genome-wide association study​.

Yuxin Wen, Jianping Yu, Xiaocao Wu, Liwei Chu, Huizan Li, Xingen Li, Xuehan Zhang, Weiyi Li, Kun Wu, Yongqiang Li and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yuxin Wen *College of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Jianping Yu *College of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China. jpyu@bua.edu.cn.
Xiaocao Wu *College of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Liwei Chu *College of Life and Health, Dalian University, Dalian, Liaoning, China.
Huizan LiCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Xingen LiCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Xuehan ZhangCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Weiyi LiCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Kun WuState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Yongqiang LiCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Runzhi LiCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Kai YangCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China.
Ping WanCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China. pingwan3@163.com.
Bo ZhaoCollege of Plant Science and Technology, Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture, Beijing, 102206, P. R. China. zhaobobac@sohu.com.

Funding

Beijing Key Laboratory of New Technique in Agricultural Application, Beijing University of Agriculture KFKT-2025011Beijing Natural Science Foundation-Beijing Municipal Education Committee KZ201710020013Beijing University of Agriculture Young Faculty Research Innovation Enhancement Program QJKC-2022050National Key Research and Development Program of China 2018YFD1000705 and 2018YFD1000700
6 · The paper itself

Abstract

backgroundIron is an essential micronutrient for both plant growth and human health and iron deficiency affects over 30% of the global population. Adzuki bean (Vigna angularis), an iron-rich legume, serves as a strategic dietary iron source to combat global iron deficiency anemia. Despite its nutritional significance, the genetic basis of iron accumulation in legumes remains unexplored.

resultsThis study addresses this gap by performing the first genome-wide association study (GWAS) targeting seed iron content in a diverse panel of 320 adzuki accessions (220 cultivated, 100 wild/semi-wild), representing seven genetic subpopulations across East Asia. Multi-environment phenotyping (2014–2019) revealed extensive iron content variation, with wild adzuki bean exhibiting significantly higher concentrations than cultivated types. No significant difference was found between landraces and improved cultivars. GWAS identified 39 quantitative trait loci (QTLs) associated with iron content, distributed across chromosomes 1 to 11, with chromosomes 3 and 10 harboring the most loci. Twelve candidate genes were prioritized within QTL intervals, including genes involved in iron transport (e.g., Vigan01g178400, homologous to iron-regulated transporter IRT1), metabolism (e.g., Vigan07g168200, a nicotinamidase aminotransferase), and regulation (e.g., Vigan03g004100, a bZIP transcription factor). Haplotype analysis of key genes uncovered functional variations: for example, VaIRT1 haplotypes with a promoter SNP showed significant differences in iron content, likely due to altered cis-acting elements affecting gene expression.

conclusionsThis study provides the first genetic blueprint for iron biofortification in adzuki bean, identifying key haplotypes and regulatory variants that modulate iron homeostasis. These findings clarify the genetic architecture of iron accumulation in adzuki bean and provide valuable resources for breeding high-iron varieties to address global iron deficiency.

Indexed as

IronQuantitative Trait LociSeedsVignaAllelesGenome-Wide Association StudyIronAdzuki beanGWASHaplotype analysisIron contentIron-regulated transporter

Identifiers

PMID41963787
PMCPMC13181979

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.