Evidence map›Paper›PMID 41963556›Full record

ArticleThe EMBO journal2026

The H3K36me3 methyltransferase SETD2 contributes to PAF1C interactions with RNA Pol II and is required for neuronal differentiation.

Christina Ambrosi, Ramon Pfaendler, Kristeli Eleftheriou, Stefan Butz, Davide Recchia, Xue Bao, Richard Cardoso da Silva, Niklas Kupfer, Ilse M Lagerwaard, Hanneke Vlaming and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The EMBO journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Christina AmbrosiDepartment of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
Ramon PfaendlerDepartment of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0003-3312-5152
Kristeli EleftheriouGenome Biology and Epigenetics, Institute of Biodynamics and Biocomplexity, Department of Biology, Utrecht University, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0001-6529-7042
Stefan ButzDepartment of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
Davide RecchiaDepartment of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0009-0004-5791-074X
Xue BaoGenome Biology and Epigenetics, Institute of Biodynamics and Biocomplexity, Department of Biology, Utrecht University, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0002-5129-0728
Richard Cardoso da SilvaGenome Biology and Epigenetics, Institute of Biodynamics and Biocomplexity, Department of Biology, Utrecht University, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0003-2767-2053
Niklas KupferGenome Biology and Epigenetics, Institute of Biodynamics and Biocomplexity, Department of Biology, Utrecht University, Utrecht, The Netherlands.ORCID http://orcid.org/0009-0000-7395-0638
Ilse M LagerwaardGenome Biology and Epigenetics, Institute of Biodynamics and Biocomplexity, Department of Biology, Utrecht University, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0002-3292-8554
Hanneke VlamingGenome Biology and Epigenetics, Institute of Biodynamics and Biocomplexity, Department of Biology, Utrecht University, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0003-1743-6428
Nina SchmolkaDepartment of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland.
Vivek BhardwajGenome Biology and Epigenetics, Institute of Biodynamics and Biocomplexity, Department of Biology, Utrecht University, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0002-5570-9338
Tuncay BaubecDepartment of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland. t.baubec@uu.nl.ORCID http://orcid.org/0000-0001-8474-6587

Funding

EC | Horizon Europe | Excellent Science | HORIZON EUROPE European Research Council (ERC) 865094Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF) 180354Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF) 183722Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF) 186012
6 · The paper itself

Abstract

Chromatin modifications are essential for mammalian development, and their aberrant deposition is associated with human disease. While the mechanisms that deposit and remove histone modifications have been largely elucidated, their roles in regulating gene activity during cellular differentiation are yet to be fully understood. Here, we performed a deletion screen to identify stage-specific requirements of chromatin regulators during neuronal differentiation of mouse embryonic stem cells. We show that the H3K36me3 methyltransferase SETD2 is required for the establishment of neuronal gene expression during late stages of differentiation but is dispensable in mature neurons. Notably, this function is independent of its histone methyltransferase activity. Instead, SETD2 promotes interactions between the PAF1 complex and elongating RNA Pol II, suggesting a role in supporting efficient transcription of neuronal genes.

Indexed as

Cell DifferentiationHistone-Lysine N-MethyltransferaseNeuronsNuclear ProteinsRNA Polymerase IIAnimalsCarrier ProteinsHistonesMiceMouse Embryonic Stem CellsCarrier ProteinsHistone-Lysine N-MethyltransferaseHistonesNuclear ProteinsRNA Polymerase IIRNA polymerase II associated factor 1, mouseSETD2 protein, mouseChromatinH3K36me3SETD2

Identifiers

PMID41963556
PMCPMC13187324

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.