Evidence map›Paper›PMID 41963388›Full record

ArticleScientific reports2026

Val109 and Ile170 contribute to channel function in Guillardia theta-derived anion channelrhodopsins.

Takanori Yokoyama, Eriko Sugano, Kitako Tabata, Taira Kakizaki, Shu Maruoka, Yuka Endo, Taku Ozaki, Tomokazu Fukuda, Lanlan Bai, Hiroshi Tomita

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Takanori Yokoyama *Laboratory of Visual Neuroscience, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
Eriko Sugano *Laboratory of Visual Neuroscience, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
Kitako TabataLaboratory of Visual Neuroscience, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
Taira KakizakiLaboratory of Visual Neuroscience, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
Shu MaruokaLaboratory of Visual Neuroscience, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
Yuka EndoLaboratory of Visual Neuroscience, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
Taku OzakiLaboratory of Biochemistry, Molecular Medical Science, Iwate University Division of Agriculture, 3-18-33 Ueda, Morioka, Iwate, 020-8550, Japan.
Tomokazu FukudaLaboratory of Cell Engineering and Molecular Genetics, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
Lanlan BaiLaboratory of Cell Engineering and Molecular Genetics, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan.
Hiroshi TomitaLaboratory of Visual Neuroscience, Molecular Medical Science, Iwate University Division of Agriculture, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan. htomita@iwate-u.ac.jp.

Funding

Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan 21K09713Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan 22H00579Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan 22K09760
6 · The paper itself

Abstract

Anion channelrhodopsins (ACRs) are widely used for optical silencing of neurons. Photocurrents and kinetics are crucial for the precise control of cell hyperpolarization. In this study, we investigated the roles of amino acid residues in each transmembrane domain of the Guillardia theta-derived anion channelrhodopsins, GtACR1 and GtACR2, to develop high-performance ACRs. Several chimaera ACRs were generated by replacing the transmembrane (TM) domains of GtACR1 with homologous counterparts of GtACR2, and photocurrents in HEK cells transduced with each variant were recorded by patch-clamp. The relationship between photocurrent and protein localisation was determined. Chimaeras of TM4, TM5, and TM6 in GtACR1 replaced with the homologous counterparts of GtACR2 showed wavelength changes and fast kinetics(τ

Indexed as

ChannelrhodopsinsCryptophytaIsoleucineAnionsHEK293 CellsHumansKineticsMolecular Dynamics SimulationAnionsChannelrhodopsinsIsoleucineanion channelrhodopsinchannelrhodopsinGtACR1GtACR2Guillaldia thetakineticsV109I

Identifiers

PMID41963388
PMCPMC13230924

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.