Evidence map›Paper›PMID 41961339›Full record

ArticleMolecular biology reports2026

Characterization of a novel panel of polymorphic microsatellite loci and the mitogenome for the Neotropical marsupial Gracilinanus agilis (Didelphimorphia: Didelphidae).

Marcela Reis Duarte da Silva, Cássia Alves Lima-Rezende, Priscilla Lóra Zangrandi, André Faria Mendonça, Renato Caparroz, Emerson Monteiro Vieira, Fernando Pacheco Rodrigues

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Marcela Reis Duarte da SilvaLaboratório de Genética e Biodiversidade, Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, CEP 70910-900, DF, Brazil.
Cássia Alves Lima-RezendeLaboratório de Genética e Biodiversidade, Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, CEP 70910-900, DF, Brazil.
Priscilla Lóra ZangrandiLaboratório de Ecologia de Vertebrados, Departamento de Ecologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, CEP 70910-900, DF, Brazil.
André Faria MendonçaLaboratório de Ecologia e Conservação Animal, Departamento de Ecologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, CEP 70910-900, DF, Brazil.
Renato CaparrozLaboratório de Genética e Biodiversidade, Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, CEP 70910-900, DF, Brazil.
Emerson Monteiro VieiraLaboratório de Ecologia de Vertebrados, Departamento de Ecologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, CEP 70910-900, DF, Brazil.
Fernando Pacheco RodriguesLaboratório de Genética e Biodiversidade, Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, CEP 70910-900, DF, Brazil. fprodrigues@unb.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicrosatellite and mitochondrial markers are important tools for investigating natural populations, enabling the assessment of their diversity, genetic structure, kinship, parentage, and reproductive biology. Although the importance of such markers is widely recognized, their use in Neotropical mammals, especially marsupials, is still limited by the scarcity of species-specific loci characterized for extant species. METHODS AND

resultsIn this study, we describe and characterize 15 polymorphic microsatellite loci and recover part of the mitochondrial genome for the Neotropical marsupial Gracilinanus agilis using massive parallel sequencing with the Illumina platform. DNA sequencing yielded a dataset comprising 2,331,566 paired reads, of which 1,434 were mapped to a previously published mitogenome of the species, resulting in a partial mitogenome of 16,098 base pairs. The microsatellite search identified a total of 4,199 loci, of which 26 pairs were tested on 30 individuals. Of these, 15 loci were successfully amplified and exhibited high levels of genetic variation, with an average of 7.73 alleles per locus, a mean observed heterozygosity of 0.63, and a mean expected heterozygosity of 0.72. Furthermore, these loci collectively provide a high paternity exclusion probability and a low identity probability.

conclusionsOur results suggest that the described marker set could be very useful for population genetic studies, evaluation of reproductive strategies (such as semelparity), and analysis of the population dynamics of this species.

Indexed as

Genome, MitochondrialMicrosatellite RepeatsAllelesAnimalsDNA, MitochondrialGenetic LociGenetic VariationHeterozygoteHigh-Throughput Nucleotide SequencingMarsupialiaOpossumsPolymorphism, GeneticSequence Analysis, DNADNA, MitochondrialGenetic parentageHigh-throughput sequencingMouse opossumPopulation genetics

Identifiers

PMID41961339
PMCPMC13068678

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