Evidence map›Paper›PMID 41961159›Full record

ArticleMammalian genome : official journal of the International Mammalian Genome Society2026

AFAP1-AS1 regulates oral squamous cell carcinoma development through the miR-93-3p/CCND1 signaling pathway.

Kuangzheng Li, Chengwei Li, Qian He, Xiaosheng Fan, Lili Xu, Yixia Jiang

Abstract read
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In one paragraph

Article in Mammalian genome : official journal of the International Mammalian Genome Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kuangzheng LiDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang, 421001, Hunan, China.
Chengwei LiDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang, 421001, Hunan, China.
Qian HeDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang, 421001, Hunan, China.
Xiaosheng FanDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang, 421001, Hunan, China.
Lili XuDepartment of Oral and Maxillofacial Surgery, The Second Affiliated Hospital, University of South China, Hengyang, 421001, Hunan, China.
Yixia JiangHengyang Medical School, University of South China, Hengyang, 421001, Hunan, China. 2012020053@usc.edu.cn.

Funding

Natural Science Foundation of Hunan Province, China 2023JJ60357
6 · The paper itself

Abstract

Actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1) has been found to be closely associated with the initiation and progression of various tumors; however, its role in oral squamous cell carcinoma (OSCC) remains unclear. AFAP1-AS1 expression in OSCC cells was detected using qRT-PCR. The regulatory effects of AFAP1-AS1 on tumor cell proliferation, migration, and invasive capabilities were systematically evaluated through CCK-8 proliferation, colony formation, wound healing, and Transwell invasion assays. Flow cytometry was employed to quantitatively analyze its impact on cell cycle progression and apoptotic. Based on bioinformatics predictions, a dual-luciferase reporter system was utilized to validate the targeting interactions among AFAP1-AS1, miR-93-3p, and CCND1. Functional rescue experiments were conducted to elucidate the functional regulatory network among them. Furthermore, a xenograft tumor model in nude mice was employed to verify in vivo the promoting effect of AFAP1-AS1 on tumor growth. AFAP1-AS1 was significantly upregulated in OSCC cells. AFAP1-AS1 knockdown inhibited the malignant phenotypes of OSCC cells. Mechanistic studies revealed that AFAP1-AS1 could target miR-93-3p and regulate CCND1 expression, thereby influencing OSCC progression. Subcutaneous tumor model in mice further confirmed the in vivo relevance of the AFAP1-AS1/miR-93-3p/CCND1 axis. AFAP1-AS1 downregulation inhibited OSCC progression through the miR-93-3p/CCND1 axis.

Indexed as

Carcinoma, Squamous CellCyclin D1MicroRNAsMouth NeoplasmsRNA, Long NoncodingSignal TransductionAnimalsApoptosisCell Line, TumorCell MovementCell ProliferationGene Expression Regulation, NeoplasticHumansMiceMice, NudeAFAP1-AS1 long noncoding RNA, humanCCND1 protein, humanCyclin D1MicroRNAsMIRN93 microRNA, humanRNA, Long NoncodingAFAP1-AS1CCND1miR-93-3pOral squamous cell carcinomaProgression

Identifiers

PMID41961159

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.