ArticleCurrent microbiology2026
Lipase Production by Pseudomonas aeruginosa ATCC 9027: Process Optimization, Kinetic Modeling and Cost Assessment.
Article in Current microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Microbial lipases are versatile biocatalysts with wide industrial relevance; however, systematic integration of media optimization, kinetic modelling, bioreactor validation, and downstream processing remains limited for Pseudomonas aeruginosa ATCC 9027. This study evaluates the lipase production potential of P. aeruginosa ATCC 9027 through media optimization, kinetic analysis, bioreactor validation, partial purification, and preliminary cost assessment. Lipase production was optimized using Response Surface Methodology (RSM), focusing on the combined influence of olive oil and dextrose. Biomass growth, product formation, and substrate utilisation kinetics were analysed using unstructured models. Batch fermentation was validated in a 1.5 L stirred-tank bioreactor and partial purification was performed. A laboratory-scale cost assessment was performed based on raw materials, equipment, and energy consumption. Optimized conditions yielded a maximum lipase activity of 0.48 U/mL (by titrimetric assay). Kinetic analysis showed that biomass growth was best described by the Logistic model (µ
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