Evidence map›Paper›PMID 41961078›Full record

ArticleCurrent microbiology2026

Genomic Insights into Mammaliicoccus sciuri from Subclinical Bovine Mastitis to Unveil Key Resistance, Virulence, Biofilm and Adaptation Traits.

Pavan Kalyan Nagaraja, Susweta Das Mitra, Devi Murugesan, Praveen Kumar Attiganahalli Muninarayanaswamy, Sujatha Geddam, Nimita Venugopal, Rituparna Tewari, Shivasharanappa Nayakvadi, Bibek Ranjan Shome, Rajeswari Shome

Abstract read
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Article in Current microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Draft genome sequence ofMicrobiology resource announcements · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Pavan Kalyan NagarajaICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, 560 064, India.
Susweta Das MitraDepartment of Biotechnology, School of Basic & Applied Sciences, Dayananda Sagar University, Bengaluru, 560078, India.
Devi MurugesanICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, 560 064, India.
Praveen Kumar Attiganahalli MuninarayanaswamyICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, 560 064, India.
Sujatha GeddamICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, 560 064, India.
Nimita VenugopalDepartment of Microbiology, M.S. Ramaiah College of Arts, Science, and Commerce, Bengaluru, 560054, India.
Rituparna TewariICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, 560 064, India.
Shivasharanappa NayakvadiICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, 560 064, India.
Bibek Ranjan ShomeICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, 560 064, India.
Rajeswari ShomeICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, 560 064, India. rajeswarishome@gmail.com.ORCID http://orcid.org/0000-0003-3559-8789

Funding

All India Indian Network for Fisheries and Animal Antimicrobial Resistance (INFAAR) funded by Indian Council of Agricultural Research, Ministry of Agriculture and Framers Welfare, Govt. of India, New Delhi IXX14760
6 · The paper itself

Abstract

The Mammaliicoccus sciuri (M. sciuri), is recognized as a reservoir of antimicrobial resistance (AMR) genes, poses challenges in the Indian dairy sector where antibiotic use is poorly regulated. This study aimed to genomically characterize M. sciuri (formerly Staphylococcus sciuri) isolates recovered from subclinical mastitis (SCM) cattle milk. A total of 128 composite (quarter-wise pooled) milk samples were collected from 199 households (HH) across 16 epiunits /villages in four blocks of Chikkaballapur district, Karnataka, India. Of these, 36 milk samples (28.13%, 36/128; 95% CI: 21.06–36.46%) were diagnosed with SCM using the California Mastitis Test (CMT) and bacteriological culture yielded 113 isolates (88.28%; 113/128; 95% CI: 81.56–92.77%) were phenotypically identified as Staph spp. Through molecular technique PCR targeting the gap gene, two isolates (1.77%; 2/113; 95% CI: 0.49–6.22%) from Hosuru and Gattamaranahalli epiunits were confirmed as M. sciuri and both isolates were mecA-positives indicating methicillin resistance. Whole genome sequencing (WGS) identified 36–37 resistance genes (mecA and blaZ), conferring resistance to β-lactams, macrolides, fluoroquinolones and aminoglycosides. Horizontal gene transfer (HGT) was evidenced by diverse mobile genetic elements (MGEs) such as SCCmec variants, insertion sequences, transposons (IS3, IS6, IS256, and IS1182) and plasmids (Rep1, Rep13, RepUS5 and RepUS43). Virulence profiling uncovered biofilm-associated genes (ica, bap) and heavy metal resistance operons (ars, cop, znu) suggesting mechanisms for environmental persistence and co-selection of resistance traits. Phylogenetic analysis of 99 global isolates revealed host-and geography-specific clustering with Indian isolates occupying distinct evolutionary niches. These findings highlights its possible role as an AMR reservoir and also in bovine mastitis.

Indexed as

Adaptation, PhysiologicalBiofilmsGenome, BacterialMastitis, BovineStaphylococcaceaeAnimalsAnti-Bacterial AgentsCattleChromosomes, BacterialDrug Resistance, BacterialFemaleGenomicsIndiaPhylogenyPlasmidsVirulenceAnti-Bacterial Agents

Identifiers

PMID41961078

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.