ArticleJournal of the American Chemical Society2026
Ribosome Heterogeneity Revealed by Complex-Up Native Mass Spectrometry and Top-Down Proteomics.
Article in Journal of the American Chemical Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Characterization of large molecular machines, such as the ribosome, under a breadth of biological states to elucidate regulatory details remains challenging. Herein, a workflow combining complex-up native mass spectrometry (nMS) with infrared multiphoton dissociation (IRMPD) and top-down proteomics (TDP) was developed to enable the rapid and direct characterization of ribosome heterogeneity. Preferential unfolding and fragmentation of rRNA by IRMPD enabled proteoform-resolved characterization of
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