Evidence map›Paper›PMID 41959666›Full record

ArticleiScience2026

Glioblastoma invasion into different organoid hosts reveals cell-intrinsic and proliferative migratory programs.

Christopher Y Akhunbay-Fudge, Bronwyn K Irving, Alima Ismail, Sabrina Samuel, Emma Smedley, Holly E Bradford, Steven Bagley, Alexander Baker, Iain M Hagan, Deena M A Gendoo and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Mesoscale maladaptation in disease organoids.Disease models & mechanisms · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Christopher Y Akhunbay-FudgeStem Cells and Brain Tumour Group, Leeds Institute of Medical Research, School of Medicine, University of Leeds, Leeds LS9 7TF, UK.
Bronwyn K IrvingStem Cells and Brain Tumour Group, Leeds Institute of Medical Research, School of Medicine, University of Leeds, Leeds LS9 7TF, UK.
Alima IsmailStem Cells and Brain Tumour Group, Leeds Institute of Medical Research, School of Medicine, University of Leeds, Leeds LS9 7TF, UK.
Sabrina SamuelStem Cells and Brain Tumour Group, Leeds Institute of Medical Research, School of Medicine, University of Leeds, Leeds LS9 7TF, UK.
Emma SmedleyStem Cells and Brain Tumour Group, Leeds Institute of Medical Research, School of Medicine, University of Leeds, Leeds LS9 7TF, UK.
Holly E BradfordStem Cells and Brain Tumour Group, Leeds Institute of Medical Research, School of Medicine, University of Leeds, Leeds LS9 7TF, UK.
Steven BagleyCancer Research UK Manchester Institute, The University of Manchester, Wilmslow Road, Manchester M20 4BX, UK.
Alexander BakerCancer Research UK Manchester Institute, The University of Manchester, Wilmslow Road, Manchester M20 4BX, UK.
Iain M HaganCancer Research UK Manchester Institute, The University of Manchester, Wilmslow Road, Manchester M20 4BX, UK.
Deena M A GendooDepartment of Cancer and Genomic Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TT, UK.
Kevin CritchleySchool of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, UK.
Ryan K MathewStem Cells and Brain Tumour Group, Leeds Institute of Medical Research, School of Medicine, University of Leeds, Leeds LS9 7TF, UK.
Heiko WurdakStem Cells and Brain Tumour Group, Leeds Institute of Medical Research, School of Medicine, University of Leeds, Leeds LS9 7TF, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Akhunbay-Fudge et al. develop two complementary single-cell profiling methods to determine glioblastoma (GB) invasion phenotypes, focusing on the influence of host organoid developmental lineage (neural versus endodermal) and cell cycle progression on GB invasion within tumor assembloids. Notably, GB cells invaded both neural and endodermal organoid hosts, whereas non-malignant adult brain cells lacked this capacity. Single-cell mRNA sequencing revealed gene expression changes in invading tumor cells and surrounding environmental assembloid cells. Concurrently, the "DyPheT" automated tracking tool enabled real-time correlation of cell cycle phases with malignant cell migration within cerebral organoids, which can be utilized for treatment response assessment, exemplified by the investigational compound RP-6306. Collectively, these approaches identify an intrinsic (cell autonomous) gene expression signature linked to GB invasion and support a "go-and-grow" paradigm by revealing a highly migratory (and RP-6306-refractory) GB subpopulation active in the G2/M phase of cell cycle.

Indexed as

CancerCell biology

Identifiers

PMID41959666
PMCPMC13059114

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.