Evidence map›Paper›PMID 41959532›Full record

ArticlebioRxiv : the preprint server for biology2026

Microbial metabolism of methotrexate produces a STAT3 signaling molecule that alleviates gut inflammation.

Juyeong Cho, Rebecca Danner, Woo Sung Kim, Jonathan Williams, Anshit Singh, Jiyoung A Han, Jonathan G Van Vranken, Allison S Walker, Timothy Rhoads, Snehal N Chaudhari

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Juyeong ChoDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Rebecca DannerDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Woo Sung KimDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Jonathan WilliamsDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Anshit SinghDepartment of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
Jiyoung A HanDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Jonathan G Van VrankenDepartment of Cell Biology, Harvard Medical School, Cambridge, MA 02115, USA.
Allison S WalkerDepartment of Chemistry, Vanderbilt University, Nashville TN 37240, USA.ORCID 0000-0001-5666-7232
Timothy RhoadsDepartment of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
Snehal N ChaudhariDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.ORCID 0000-0003-4389-3366

Funding

Biotechnology Training ProgramT32GM135066 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SCOTT M. COYLE, BRIAN G FOX · 2020 to 2026
$7.0M
Machine learning approaches for the discovery, repurposing, and optimization of natural products with therapeutic potential - Supplement to support grad training of Adrian RussR35GM146987 · NIGMS · VANDERBILT UNIVERSITY · PI Allison Sara Walker · 2022 to 2026
$2.4M
Chemistry-Biology Interface Training ProgramT32GM152341 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Helen E. Blackwell · 2024 to 2026
$1.6M
Elucidating the role of the microbiome in inducing gut permeability and inflammationR00DK128503 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI Snehal N. Chaudhari · 2024 to 2026
$727k
NIDDK NIH HHS R00 DK128503NIGMS NIH HHS R35 GM146987NIGMS NIH HHS T32 GM135066NIGMS NIH HHS T32 GM152341
6 · The paper itself

Abstract

Methotrexate (MTX) therapy in inflammatory bowel disease (IBD) is often limited by inter-individual variability in clinical response and adverse effects. Gut microbiota contribute to MTX therapeutic response and toxicity by metabolizing MTX and altering its bioavailability. However, how inflammation alters microbial MTX metabolism and how its metabolites influence the host remain poorly understood. Here, we identify

Identifiers

PMID41959532
PMCPMC13060975

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.