Evidence map›Paper›PMID 41959191›Full record

ArticlebioRxiv : the preprint server for biology2026

Mechanistic insights into the color transformation of a non-FRET substrate for RNase activity detection.

Sohyun Kim, Soonwoo Hong, Jada N Walker, Yujie He, Anh-Thu Nguyen, Wei-Ru Chen, Saeed Seifi, Yuan-I Chen, Yu-An Kuo, Jennifer S Brodbelt and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sohyun KimDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.
Soonwoo HongDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.
Jada N WalkerDepartment of Chemistry, The University of Texas at Austin, Texas, 78712, USA.
Yujie HeDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.
Anh-Thu NguyenDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.
Wei-Ru ChenDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.
Saeed SeifiDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.
Yuan-I ChenDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.
Yu-An KuoDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.
Jennifer S BrodbeltDepartment of Chemistry, The University of Texas at Austin, Texas, 78712, USA.
Hsin-Chih YehDepartment of Biomedical Engineering, The University of Texas at Austin, Texas, 78712, USA.

Funding

Developing an ultrafast fluorescence lifetime imaging ophthalmoscopy system for retinal imagingR21EY033106 · NEI · UNIVERSITY OF TEXAS AT AUSTIN · PI YEH, HSIN-CHIH · 2021 to 2022
$399k
NEI NIH HHS R21 EY033106
6 · The paper itself

Abstract

DNA-templated silver nanoclusters (DNA/AgNCs) have created a new class of non-FRET DNase substrates, termed Subak, that exhibits a color change upon DNase digestion. Although Subak substrates offer advantages such as ratiometric readouts and low manufacturing costs over traditional FRET substrates, the mechanism governing AgNC color switching remains unclear. Here, using a site-specific cleavage strategy, we identify color-switching hotspots and demonstrate that AgNC transformation can be controlled by the cleavage positions within the nucleic acid host. Our data support a cleavage-driven reorganization of the AgNC coordination environment, converting a non-emissive precursor into a red-emitting cluster, rather than direct enzyme-cluster interactions. Leveraging this insight, we engineer rSubak, an RNA-incorporated Subak that displays 95 nm red shift (530 to 625 nm) upon RNase cleavage. In amplification-free CRISPR/Cas13 assays for SARS-CoV-2, influenza A (A/H5N1), and measles viruses (MV) detection, rSubak achieved a limit of detection of 0.3 pM, superior to that of the commercial RNaseAlert (~250 pM). Collectively, our results establish Subak as a generalizable, non-FRET platform for sensitive ratiometric reporting the activities of diverse nucleases.

Identifiers

PMID41959191
PMCPMC13060967

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.