Evidence map›Paper›PMID 41959065›Full record

ArticlebioRxiv : the preprint server for biology2026

Critical roles of MCM8 in meiotic recombination during mouse spermatogenesis.

Lava Kumar Surarapu, Kevin Tilton, Maria Rosaria Dello Stritto, Ananya Acharya, Andrea Marton Menendez, Min Lu, Najma Shaheen, Shun Liang, Mythri Iyer, Petr Cejka and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lava Kumar SurarapuDepartment of Genetics and Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.
Kevin TiltonDepartment of Genetics and Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.
Maria Rosaria Dello StrittoInstitute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
Ananya AcharyaInstitute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
Andrea Marton MenendezGenetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD, USA.
Min LuMolecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Najma ShaheenGenetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD, USA.
Shun LiangDepartment of Genetics and Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.
Mythri IyerDepartment of Genetics and Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.
Petr CejkaInstitute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
Florencia PrattoGenetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD, USA.
Devanshi JainDepartment of Genetics and Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, USA.ORCID 0000-0002-5027-0549

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI SELWYN M VICKERS · 1985 to 2026
$347.4M
Mechanism and regulation of meiotic recombinationR35GM118092 · NIGMS · SLOAN-KETTERING INST CAN RESEARCH · PI Scott Keeney · 2016 to 2026
$7.0M
Regulatory mechanisms of meiotic entry and progressionR35GM147130 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI Devanshi Jain · 2022 to 2026
$1.9M
NCI NIH HHS P30 CA008748NIGMS NIH HHS R35 GM118092NIGMS NIH HHS R35 GM147130
6 · The paper itself

Abstract

Meiotic DNA double-strand break (DSB) formation and repair by homologous recombination is crucial for ensuring proper chromosome segregation. In mice, the mini-chromosome maintenance family protein, MCM8, has been proposed to function in meiotic recombination and its loss leads to infertility, but the underlying mechanisms are poorly understood. Here we used cytological and genomic assays to infer the role of MCM8 during meiotic recombination in mouse spermatocytes. We show that MCM8-deficient spermatocytes exhibit increased levels of SPO11-dependent DSBs at recombination hotspots during early prophase. DSBs are resected normally and accumulate strand-exchange proteins. However, downstream recombination intermediates are barely detected and recombination intermediate-associated MutSgamma foci do not form efficiently. Consistent with a role in early recombination intermediate processing, MCM8 binds to displacement loop (D-loop) structures

Identifiers

PMID41959065
PMCPMC13060065

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.