Evidence map›Paper›PMID 41957682›Full record

ArticleCell & bioscience2026

Integrating single-cell and spatial transcriptomics to reveal the spatiotemporal dynamics of retinal cells during ischemia-reperfusion injury progression in rats.

Yijia Huang, Junhong Guo, Fei Yao, Bingkai Feng, Di Gong, Kuanrong Dang, Tingyu Hu, Simin Deng, Yong Liu, Chunyan Tan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell & bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. PEBP1 Regulates Ferroptosis in Acute Glaucoma: Targeted Therapy Using Engineered Exosomes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yijia Huang *Shenzhen Eye Institute of Shenzhen Eye Hospital, Jinan University, Shenzhen, Guangdong, China.
Junhong Guo *Shenzhen Eye Institute of Shenzhen Eye Hospital, Jinan University, Shenzhen, Guangdong, China.
Fei Yao *Eye Center of Xiangya Hospital, Central South University, Changsha, Hunan, China.
Bingkai FengShenzhen Eye Institute of Shenzhen Eye Hospital, Jinan University, Shenzhen, Guangdong, China.
Di GongShenzhen Eye Institute of Shenzhen Eye Hospital, Jinan University, Shenzhen, Guangdong, China.
Kuanrong DangShenzhen Eye Institute of Shenzhen Eye Hospital, Jinan University, Shenzhen, Guangdong, China.
Tingyu HuShenzhen Eye Medical Center of Shenzhen Eye Hospital, Southern Medical University, Shenzhen, Guangdong, China.
Simin DengShenzhen Eye Institute of Shenzhen Eye Hospital, Jinan University, Shenzhen, Guangdong, China.
Yong LiuShenzhen Eye Institute of Shenzhen Eye Hospital, Jinan University, Shenzhen, Guangdong, China.
Chunyan TanInstitute of Biopharmaceutical and Health Engineering, Tsinghua University, Shenzhen, Guangdong, China. tancy@sz.tsinghua.edu.cn.
Jiantao WangShenzhen Eye Institute of Shenzhen Eye Hospital, Jinan University, Shenzhen, Guangdong, China. jiantaowang65@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Retinal ischemia–reperfusion (I/R) injury is a key pathological process in retinal-associated diseases such as glaucoma. However, the spatiotemporal dynamics and cell interactions during this injury are not fully understood. This study integrated single-cell RNA sequencing and spatial transcriptomics to map retinal cell responses in a rat I/R injury model at acute, subacute, and chronic stages. We identified 14 cell populations and tracked gene expression changes over time. Retinal ganglion cells (RGCs) showed activation of apoptotic, autophagic, and oxidative stress pathways within 24 h. Spatial analysis revealed RGCs co-localized with Müller glial and microglial cells in the optic nerve and inner retina, with RGC death driven by ligand-receptor interactions (e.g., Ncam1-Ncam1, App-Sorl1). Transcription factors (Sox9/Sox8) in Müller cells regulated TNF/JAK-STAT signaling, while Alzheimer’s-related Mapt exhibited spatiotemporal specificity in RGCs, suggesting shared neurodegenerative pathways. This study constructed a panoramic spatiotemporal dynamic map of retinal I/R injury progression, providing important clues for the development of potential therapeutic targets for glaucoma and related retinal diseases.

Indexed as

Cell and tissue heterogeneityGlaucomaRetinal ischemia–reperfusion injurySingle-cell transcriptome sequencingSpatial transcriptome sequencing

Identifiers

PMID41957682
PMCPMC13214335

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.