Sequential extraction and organosolv pretreatment of halophytes: unlocking biomass recalcitrance for bio-based production.

Maxwel Monção, Ahmed Al-Dubai, Aadila Cayenne, Hinrich Uellendahl, Ulrika Rova, Paul Christakopoulos, Leonidas Matsakas

Abstract read

In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Maxwel MonçãoBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971 87, Luleå, Sweden.

Ahmed Al-DubaiBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971 87, Luleå, Sweden.

Aadila CayenneFaculty of Mechanical and Process Engineering and Maritime Technologies, Flensburg University of Applied Sciences, Kanzleistr. 91-93, 24943, Flensburg, Germany.

Hinrich UellendahlFaculty of Mechanical and Process Engineering and Maritime Technologies, Flensburg University of Applied Sciences, Kanzleistr. 91-93, 24943, Flensburg, Germany.

Ulrika RovaBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971 87, Luleå, Sweden.

Paul ChristakopoulosBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971 87, Luleå, Sweden.

Leonidas MatsakasBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971 87, Luleå, Sweden. leonidas.matsakas@ltu.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The growing demand for sustainable bioresources has given prominence to lignocellulosic biomass as a possible alternative. Halophytes offer a specific advantage in this context, because they can grow on non-arable land with minimal freshwater requirements. To our knowledge, this is the first study to integrate organosolv fractionation to either Soxhlet extraction (SLE) or subcritical water extraction, (SWE) at 140 °C, as pretreatment strategies, into a single sequential process applied to halophyte biomass allowing the recovery of bioactive molecules from halophyte biomass. The spent fibers underwent organosolv fractionation under varied process conditions (180-200 °C, 15-60 min, 40-60% v/v ethanol) and were subsequently evaluated for bioenergy production via anaerobic digestion. Organosolv fractionation generates separate lignin and hemicellulose streams, which can be further converted into value-added products. Either extraction methodology effectively facilitated subsequent biomass fractionation. SLE-pretreated fibers attained > 96.4% hemicellulose removal in 87.5% of the tested conditions and 78.1% delignification. SWE-pretreated fibers exhibited slightly lower hemicellulose removal at higher (60% v/v) ethanol content and 69.3% delignification. Crucially, SWE fibers demonstrated superior bioenergy potential, achieving a methane yield of 336 mL-CH

Indexed as

BiofuelsBiomassSalt-Tolerant PlantsChemical FractionationEthanolLigninPolysaccharidesBiofuelsEthanolhemicelluloseLigninlignocellulosePolysaccharidesAnaerobic digestionBiorefineryHalophyteOrganosolv fractionationSalicornia

Identifiers

PMID41957173

DOI10.1038/s41598-026-46584-w

PMCPMC13076653

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.