ArticleScientific reports2026
Biodegradation of chlorpyrifos by the newly isolated Escherichia fergusonii and Clostridium bifermentans: Identification and growth optimization for bioremediation.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Chlorpyrifos, widely used in Ethiopia despite restricted official status, contaminates soil and water, posing environmental and health risks. Microbial bioremediation offers a natural, eco-friendly solution for its removal from contaminated sites. This study aimed to identify bacteria from Meki and Wonji-shoa agricultural soil, capable of degrading CP and optimize their growth conditions, such as pH, temperature, and CP concentration using enrichment technique. With a UV-VIS Spectrophotometer (600 nm), for the strains growth response and (290 nm) for degradation of chlorpyrifos were observed every two days. Based on their tolerance to high CP concentration and degradation capacity, two isolates out of eight potential strains were selected, identified using biochemical characterization and 16 S r RNA sequencing to be Escherichia fergusonii LB50 and Clostridium bifermentans PS1. The bacterial strains isolated in the current study were not previously reported in CP degradation. Their efficacy of CP degradation further confirmed by GC-MS. These isolates were able to degrade 59.34% (Escherichia fergusonii) and 70.82% (Clostridium bifermentans) of the initial concentration of chlorpyrifos (50 mg L
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