ArticleJournal of the American Chemical Society2026
Bimodal Cholesterol for Correlative In-Cell DNP Solid-State NMR and Confocal Microscopy of the Plasma Membrane.
Article in Journal of the American Chemical Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cholesterol plays a pivotal role in defining the structure and signaling function of plasma membrane lipid rafts, yet direct nanoscale measurements of cholesterol-rich domains in intact cells that enable atomic scale analysis remains challenging. Here, we present AsymPol-Chol-AF647, a bimodal cholesterol-based probe that integrates a biradical (AsymPol) for Dynamic Nuclear Polarization (DNP) with a fluorescent dye (AF647), enabling correlative in-cell DNP solid-state NMR and confocal fluorescence microscopy of the plasma membrane. The cholesterol anchor ensures insertion into the plasma membrane, providing a spatially defined delivery of the polarizing agent. Confocal imaging in live cells confirms plasma membrane localization and reveals concentration-dependent perturbations to lipid raft integrity, with optimal conditions at 0.19 nmol/10
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