Evidence map›Paper›PMID 41953720›Full record

ArticleMaterials today. Bio2026

Cholesterol analogs modulate lipid nanoparticle performance for mRNA delivery after lyophilization and enable ocular disease therapy.

Ting Wang, Wanqi Li, Jianyang Chen, Sitian Cheng, Min Gao, Chengyu Jiang, Chen Jiang, Bingyi Hu, Tzu-Cheng Sung, Akon Higuchi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Materials today. Bio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ting WangState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Wanqi LiState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Jianyang ChenState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Sitian ChengState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Min GaoState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Chengyu JiangState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Chen JiangState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Bingyi HuState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Tzu-Cheng SungState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.
Akon HiguchiState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, Wenzhou, Zhejiang, 325027, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipid nanoparticles (LNPs) are essential nucleic acid delivery carriers, with cholesterol being crucial for their structural integrity and intracellular transport. Although C24-alkyl phytosterols (cholesterol analogs) promote gene transfection in HeLa cells, their broader impact on mRNA-LNP performance across organs and organ-derived cell lines remains unknown. We investigated the effects of C24-alkyl phytosterols on mRNA-LNP morphology, stability, transfection efficiency, and

Indexed as

Cholesterol analogsLipid nanoparticlesLyophilizationOcular gene therapyTransfection efficiency

Identifiers

PMID41953720
PMCPMC13053809

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.