Evidence map›Paper›PMID 41953016›Full record

ArticleFrontiers in immunology2026

A murine model of sepsis induces age- and sex-specific chromatin remodeling in myeloid-derived suppressor cells.

Angel M Charles, Christine E Rodhouse, Dijoia B Darden, Marie-Pierre L Gauthier, Mingqi Zhou, Miguel Hernandez-Rios, Dayuan Wang, Gemma Casadesus, Letitia Bible, Alicia M Mohr and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

21 authors.

Angel M CharlesSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Christine E RodhouseSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Dijoia B DardenSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Marie-Pierre L GauthierDepartment of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, United States.
Mingqi ZhouDepartment of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, United States.
Miguel Hernandez-RiosSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Dayuan WangSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Gemma CasadesusDepartment of Pharmacy and Therapeutics, University of Florida College of Medicine, Gainesville, FL, United States.
Letitia BibleSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Alicia M MohrSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Feifei XiaoSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Guoshuai CaiSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Jason O BrantDepartment of Biostatistics, College of Public Health & Health Professions, University of Florida, Gainesville, FL, United States.
Shannon M WalletDepartment of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, United States.
Clayton E MathewsDepartment of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, United States.
Lyle L MoldawerSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Paramita ChakrabartyDepartment of Neuroscience, University of Florida College of Medicine, Gainesville, FL, United States.
Rhonda L BacherDepartment of Biostatistics, College of Public Health & Health Professions, University of Florida, Gainesville, FL, United States.
Philip A EfronSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Robert MaileSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.
Michael P KladdeSepsis and Critical Illness Research Center, Department of Surgery, College of Medicine, University of Florida, Gainesville, FL, United States.

Funding

Molecular Biology in Burns and TraumaT32GM008721 · NIGMS · UNIVERSITY OF FLORIDA · PI Philip A Efron · 1999 to 2026
$5.0M
Resolving single-cell analysis challenges via data-driven decision frameworks and novel statistical methodsR35GM146895 · NIGMS · UNIVERSITY OF FLORIDA · PI Rhonda Bacher · 2022 to 2026
$1.9M
Multi-modal rescue of pulmonary NRF2-insufficiency after burn and burn + inhalation injury to regulate innate immune dysfunctionR01GM146134 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Robert Maile · 2022 to 2026
$1.6M
Sepsis and the Systemic Cytokine Storm in Aging and Alzheimer Disease ModelsR01NS128626 · NINDS · UNIVERSITY OF FLORIDA · PI PARAMITA CHAKRABARTY, Philip A Efron · 2025 to 2026
$1.1M
NIGMS NIH HHS R01 GM146134NIGMS NIH HHS R35 GM146895NIGMS NIH HHS T32 GM008721NINDS NIH HHS R01 NS128626
6 · The paper itself

Abstract

Introduction: Sepsis survivors frequently develop long-term immune dysfunction, but the epigenetic mechanisms underlying persistent myeloid suppression remain unclear. Myeloid-derived suppressor cells (MDSCs), whose function is shaped by host age and sex, are key contributors to post-sepsis immune dysregulation. Methods: Here, we present a high-resolution epigenetic map targeting gene promoters of MDSCs after sepsis and daily chronic stress using MAPit-FENGC, a single-molecule assay that simultaneously profiles DNA methylation and chromatin accessibility. In a clinically relevant murine model, including young and older adult male and female mice, splenic MDSCs were isolated for MAPit-FENGC and single-cell RNA sequencing. Results: Unsupervised clustering identified nine promoter classes reflecting chromatin dynamics: age- and sex-dependent sepsis-induced opening (Classes 1-4), persistent closure with varying levels of DNA methylation (Classes 5-7), and constitutive openness post-sepsis (Classes 8, 9). Transcriptomic profiling corroborated these promoter states, linking accessibility with gene expression. Conclusions: These findings define promoter-level epigenetic classes across a targeted locus panel in splenic CD11b

Indexed as

Chromatin Assembly and DisassemblyMyeloid-Derived Suppressor CellsSepsisAge FactorsAnimalsDisease Models, AnimalDNA MethylationEpigenesis, GeneticFemaleMaleMiceMice, Inbred C57BLPromoter Regions, GeneticSex Factorsagingchromatin accessibilityDNA methylationepigeneticsmyeloid-derived suppressor cellsprecision medicinesepsissex

Identifiers

PMID41953016
PMCPMC13053262

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.