Evidence map›Paper›PMID 41952823›Full record

ArticleFrontiers in public health2026

LAMP-LFD: establishment and evaluation of a new diagnostic platform for SARS-CoV-2 rapid detection.

Qizhi Diao, Sha Tang, Xin Liu, Jiajia Li, Xiangmin Zhou, Yuanyu Chen, Qiongyuan Zhang, Fangyu Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in public health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Rapid Visual Detection ofJournal of fungi (Basel, Switzerland) · 2026
    Article
  2. Rapid and sensitive detection ofFrontiers in cellular and infection microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qizhi DiaoDepartment of Clinical Laboratory Medicine, Sanya Women and Children's Hospital Affiliated to Hainan Medical University, Sanya, China.
Sha TangDepartment of Clinical Laboratory Medicine, The First Affiliated Hospital of Hainan Medical University, Haikou, China.
Xin LiuDepartment of Clinical Laboratory Medicine, Hainan Branch, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Sanya, China.
Jiajia LiDepartment of Clinical Laboratory Medicine, Hainan Branch, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Sanya, China.
Xiangmin ZhouDepartment of Clinical Laboratory Medicine, Hainan Branch, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Sanya, China.
Yuanyu ChenDepartment of Clinical Laboratory Medicine, Hainan Branch, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Sanya, China.
Qiongyuan Zhang *Department of Clinical Laboratory Medicine, University-Town Hospital of Chongqing Medical University, Chongqing, China.
Fangyu Yang *Department of Clinical Laboratory Medicine, University-Town Hospital of Chongqing Medical University, Chongqing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: This study aimed to establish a rapid detection method for SARS-CoV-2 by integrating loop-mediated isothermal amplification (LAMP) with lateral flow dipstick (LFD) technology. Method: Specific primers targeting the nucleocapsid (N) gene and open reading frame 1ab (ORF1ab) gene of SARS-CoV-2 were designed and labeled with 6-FAM and biotin, respectively. After systematically optimizing key reaction parameters, including primer selection, primer concentration, and LAMP reaction time, the sensitivity, specificity, and clinical applicability of the method were evaluated. Results: The proposed LAMP-LFD assay enables rapid detection within 30 min with high specificity, accurately identifying the N and ORF1ab genes of SARS-CoV-2 without cross-reactivity with influenza A hemagglutinin gene, influenza B neuraminidase gene, and respiratory syncytial virus M gene. The limit of detection reached 1.892 × 10 Discussion: This study provides technical support for the surveillance and control of SARS-CoV-2 infection.

Indexed as

COVID-19Molecular Diagnostic TechniquesNucleic Acid Amplification TechniquesSARS-CoV-2HumansRapid Diagnostic TestsSensitivity and Specificitylateral flow dipstickloop-mediated isothermal amplificationrapid detectionSARS-CoV-2the N gene and The ORF1ab gene

Identifiers

PMID41952823
PMCPMC13055591

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.