Evidence map›Paper›PMID 41952231›Full record

ArticleLaboratory animal research2026

Susceptibility and transcriptional characteristics of inbred C57BL/6J and outbred ICR mice in CDAHFD-induced MASH models.

Jing Zhou, Bingyi Li, Ziyi Guo, Bing Zhou, Yan Lu, Lian Zhu, Jin Lu, Jian Wang, E Wang, Yao Li

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Article in Laboratory animal research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

10 authors.

Jing Zhou *Department of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai, 200025, China.
Bingyi Li *Department of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai, 200025, China.
Ziyi Guo *Department of Endocrinology and Metabolism, Minhang Hospital, Fudan University, Shanghai, China.
Bing ZhouInstitute of Metabolism and Regenerative Medicine, Shanghai Sixth People's Hospital to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yan LuInstitute of Metabolism and Regenerative Medicine, Shanghai Sixth People's Hospital to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Lian ZhuDepartment of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai, 200025, China.
Jin LuDepartment of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai, 200025, China.
Jian WangDepartment of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai, 200025, China.
E WangDepartment of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai, 200025, China. wange@shsmu.edu.cn.ORCID http://orcid.org/0009-0005-1404-0032
Yao LiDepartment of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai, 200025, China. yao.li@shsmu.edu.cn.

Funding

Key Technologies Research and Development Program 2023YFA1800803National Natural Science Foundation of China 82070887National Natural Science Foundation of China 82370572
6 · The paper itself

Abstract

backgroundMetabolic dysfunction-associated steatohepatitis (MASH) is a progressive fibrotic liver disease, the underlying mechanisms of which have not been fully elucidated. Most MASH research relies on diet-induced models, particularly inbred mouse strains such as C57BL/6J. Although inbred strains are commonly used, outbred mice more accurately reflect the genetic diversity of human populations. It was reported that ICR mice were resistant to developing MASH after high-fat diet feeding. However, the effects of choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) on ICR mice remain unexplored. The CDAHFD is a widely adopted diet-induced model for MASH. In this study, C57BL/6J and ICR mice were fed either a normal diet (ND) or a CDAHFD for 10 weeks. Metabolism-related phenotypes and liver histology assessments were conducted to establish MASH models. RNA transcriptome sequencing of liver samples was performed to identify differentially expressed genes, which were then aligned to the human MASH transcriptome.

resultsWe successfully established MASH models via CDAHFD in both C57BL/6J and ICR mouse strains. ICR mice presented transcriptional profiles comparable to those of the C57BL/6J strain and effectively replicated the inflammatory and fibrotic features observed in patients with MASH.

conclusionsThis study revealed that ICR mice are as suitable as inbred C57BL/6J mice for CDAHFD-induced MASH models.

Indexed as

CDAHFDInbredMASHMouse strainOutbred

Identifiers

PMID41952231
PMCPMC13059155

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