ArticlePharmaceutical research2026
An Innovative Multifaceted Approach to Enhance the Sensitivity of a Target Engagement ELISA.
Article in Pharmaceutical research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
Target engagement (TE) assessments often come with bioanalytical challenges, particularly with low baseline level targets like cytokines and chemokines. Commercial immunoassay kits often cannot meet the requirements of sensitivity and drug interference. We present a novel methodology that significantly enhances the sensitivity of a conventional sandwich enzyme-linked immunosorbent assay (ELISA) for TE assessment. To increase sensitivity, various strategies were applied, including the combined use of U-bottomed assay plates and Intelligent Multifunctional Analytical Plates (IMAPlate™). Lowering volumes of enzyme substrate and stop solution in U-bottomed plate led to a concentrated color solution. The unique bottom-less "well" of the IMAPlate™ was utilized to measure the intensified color solution, resulting in a significantly magnified signal. The use of Pluronic
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