Evidence map›Paper›PMID 41951557›Full record

ArticleAustralian endodontic journal : the journal of the Australian Society of Endodontology Inc2026

Impact of EDTA Formulations and Activation Methods on Stem Cell Response, Antimicrobial Activity and Dentine Integrity: An In Vitro Study.

Letycia Accioly Simões Coelho, Laura Ferreira Almeida, Ester Alves Ferreira Bordini, Fernanda Balestrero Cassiano, Mirela Cesar Barros, Talita Tartari, Flaviana Bombarda de Andrade, Diana Gabriela Soares Dos Passos, Marco Antonio Hungaro Duarte

Abstract read
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In one paragraph

Article in Australian endodontic journal : the journal of the Australian Society of Endodontology Inc, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Letycia Accioly Simões CoelhoBauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.ORCID https://orcid.org/0000-0002-0751-6988
Laura Ferreira AlmeidaBauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.ORCID https://orcid.org/0000-0002-1675-9383
Ester Alves Ferreira BordiniBauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.ORCID https://orcid.org/0000-0002-4178-5794
Fernanda Balestrero CassianoBauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.ORCID https://orcid.org/0000-0002-2336-876X
Mirela Cesar BarrosBauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.ORCID https://orcid.org/0000-0002-4711-3841
Talita TartariPiracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil.ORCID https://orcid.org/0000-0003-4372-0158
Flaviana Bombarda de AndradeBauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.ORCID https://orcid.org/0000-0002-1238-2160
Diana Gabriela Soares Dos PassosBauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.ORCID https://orcid.org/0000-0002-1485-6104
Marco Antonio Hungaro DuarteBauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.ORCID https://orcid.org/0000-0003-3051-737X

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 125017/2024-3Conselho Nacional de Desenvolvimento Científico e Tecnológico 403499/2022-4Conselho Nacional de Desenvolvimento Científico e Tecnológico 443991/2024-3Fundação de Amparo à Pesquisa do Estado de São Paulo 2023/01051-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2024/18863-0
6 · The paper itself

Abstract

This study evaluated the effects of 17% trisodium EDTA and 15% disodium EDTA (EDTAd), with or without activation, on SCAP response, antimicrobial activity and dentine chemical alterations. SCAP viability (Live/Dead) and metabolic activity (Alamar Blue) were assessed, along with antibiofilm and intratubular disinfection against Enterococcus faecalis and Streptococcus mutans, after irrigation with EDTA or EDTAd delivered by conventional irrigation (CI), ultrasonic activation (UA) or diode laser activation (LA). Dentine alterations were analysed by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). EDTA preserved higher SCAP metabolic activity, particularly at early time points (p < 0.05), while cytotoxicity did not differ among groups. Antibiofilm and intratubular antimicrobial effects were similar across irrigants and activation methods (p > 0.05). EDTAd caused greater alterations in the amide III/phosphate ratio (p < 0.05), with increased smear-layer removal and surface irregularities. EDTA showed greater biological compatibility and fewer dentine alterations, whereas EDTAd induced more pronounced structural changes without antimicrobial differences.

Indexed as

DentinEdetic AcidRoot Canal IrrigantsStem CellsBiofilmsCell SurvivalEnterococcus faecalisHumansIn Vitro TechniquesLasers, SemiconductorMicroscopy, Electron, ScanningSmear LayerSpectroscopy, Fourier Transform InfraredStreptococcus mutansEdetic AcidRoot Canal Irrigantsantimicrobial activitybiofilmregenerative endodonticsroot canal irrigationstem cells from apical papilla

Identifiers

PMID41951557
PMCPMC13436179

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.