Evidence map›Paper›PMID 41950756›Full record

ArticlePoultry science2026

Oral administration of recombinant Lactobacillus plantarum co expressing FimB, CnaA, NetB and FBA antigens targeting chicken dendritic cells provides effective protection against necrotizing enteritis in broilers.

Tianrui Yang, Tong Wang, Gerui Zhang, Qiyu Guo, Yuxi Zhang, Yupeng Gao, Yan Sun, Yuhang Zhang, Xin Wen, Xin Lu and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Tianrui YangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Tong WangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Gerui ZhangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Qiyu GuoCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Yuxi ZhangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Yupeng GaoCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Yan SunCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Yuhang ZhangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Xin WenCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Xin LuCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Yao XuCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Yijie WangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Linhai DuCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Haibin HuangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Shengnan ZouCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China; Ministry of Education Key Lab for Avian Preventive Medicine; Yangzhou University, China.
Zhannan WangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address: wangzn@jlau.edu.cn.
Chunfeng WangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address: wangchunfeng@jlau.edu.cn.
Yanlong JiangCollege of Animal Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address: yanlong.jiang@jlau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Necrotic enteritis (NE), an acute intestinal disease of broilers caused by Clostridium perfringens, poses a significant threat to global poultry production. The ban on antibiotic growth promoters in feed, particularly in the EU since 2006, has exacerbated this problem, leading to a resurgence of NE and substantial economic losses. To address this challenge, we developed a novel probiotic-based oral vaccine strategy. We engineered three recombinant Lactobacillus plantarum NC8 strains, NC8(pYL560) expressing three secreted C. perfringens antigens (FimB, NetB, CnaA), NC8(pYL594) expressing a fourth surface-displayed antigen (FBA), and NC8(pYL595), which co-delivers these four antigens with a dendritic cell (DC)-targeting nanobody (phi74) fused with FBA. In vitro, the DC-targeting strain NC8(pYL595) showed superior uptake by chicken bone marrow-derived DCs compared to the non-targeting strain (78.4% vs. 46.9%, p < 0.01), along with enhanced adhesion, invasion, and the ability to promote DC maturation (upregulating CD83, CD86, CCL5, CCR7). After a total of six dose immunization, both NC8(pYL594) and NC8(pYL595) could moderately elevate the antigen specific serum IgG, intestinal IgG, IgA and IgM antibodies compared with NC8(pYL560), especially the intestinal IgA and IgG. In addition, the splenic cellular proliferation and productions of intracellular IL-4 and IFN-γ were also moderately increased in both NC8(pYL594) and NC8(pYL595) groups. Most importantly, in a broiler challenge model, oral immunization with either NC8(pYL560), NC8(pYL594) or NC8(pYL595), especially NC8(pYL595), significantly reduced averate intestinal lesions to 1.6, 1.5 and 0.8, respectively, compared with the mock control (lesion score=3.67) and empty vector control (lesion score=3.125). In addition, immunization with NC8(pYL595) resulted in dramatically decreased disease positive rates to 60%, compared with 77.7% in NC8(pYL560) group and 87.5% in NC8(pYL594) group. In conclusion, our findings demonstrate that the integration of multi-antigen co-expression and DC-targeting synergistically enhances the immunogenicity and protective effects of an oral lactobacilli vaccine, offering a promising antibiotic-free strategy for NE control.

Indexed as

Antigens, BacterialBacterial VaccinesChickensClostridium InfectionsClostridium perfringensEnteritisLactiplantibacillus plantarumPoultry DiseasesProbioticsAdministration, OralAnimalsDendritic CellsMicroorganisms, Genetically-ModifiedAntigens, BacterialBacterial VaccinesClostridium perfringensDendritic cell targetingLactobacillus plantarumMultivalent antigenNecrotic enteritis

Identifiers

PMID41950756
PMCPMC13090651

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