Evidence map›Paper›PMID 41950010›Full record

ArticleSTAR protocols2026

Protocol for stable isotope tracing of primary human peripheral blood mononuclear cells.

Samantha N Hart, Holden Williams, William B Inabnet, Joshua P Steiner, Varun Jain, Philip A Kern, Cecily R Wood, Barbara S Nikolajczyk, Lance A Johnson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Samantha N HartDepartment of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, 741 S Limestone, Lexington, KY 40536, USA; Barnstable Brown Diabetes and Obesity Research Center, University of Kentucky, 1000 S Limestone, Lexington, KY 40536, USA. Electronic address: snda236@uky.edu.
Holden WilliamsDepartment of Physiology, College of Medicine, University of Kentucky, 741 S Limestone BBSRB B243, Lexington, KY 40536, USA.
William B InabnetDepartment of Surgery, University of Kentucky, 800 Rose Street, Lexington, KY 40536, USA.
Joshua P SteinerDepartment of Surgery, University of Kentucky, 800 Rose Street, Lexington, KY 40536, USA.
Varun JainDepartment of Surgery, University of Kentucky, 800 Rose Street, Lexington, KY 40536, USA.
Philip A KernBarnstable Brown Diabetes and Obesity Research Center, University of Kentucky, 1000 S Limestone, Lexington, KY 40536, USA; The Department of Internal Medicine, Division of Endocrinology, 900 S Limestone CTW 521, Lexington, KY 40536, USA.
Cecily R WoodDepartment of Physiology, College of Medicine, University of Kentucky, 741 S Limestone BBSRB B243, Lexington, KY 40536, USA.
Barbara S NikolajczykBarnstable Brown Diabetes and Obesity Research Center, University of Kentucky, 1000 S Limestone, Lexington, KY 40536, USA; Department of Pharmacology and Nutritional Sciences, College of Medicine, University of Kentucky, 780 Rose Street MS305, Lexington, KY 40536, USA. Electronic address: bnik@uky.edu.
Lance A JohnsonDepartment of Physiology, College of Medicine, University of Kentucky, 741 S Limestone BBSRB B243, Lexington, KY 40536, USA; Sanders Brown Center on Aging, College of Medicine, University of Kentucky, 789 S Limestone, Lexington, KY 40536, USA; Department of Neuroscience, University of Kentucky, 760 Press Ave, Lexington, KY 40536, USA. Electronic address: johnson.lance@uky.edu.

Funding

University of Kentucky Diabetes Prevention COBRE (UK-DPC)P20GM156679 · NIGMS · UNIVERSITY OF KENTUCKY · PI SIMON J FISHER, Barbara Nikolajczyk · 2025 to 2026
$6.8M
The impact of insulin sensitivity on the potential of metformin to delay age-related inflammationR01AG079525 · NIA · UNIVERSITY OF KENTUCKY · PI NIKOLAJCZYK, BARBARA · 2022 to 2025
$2.3M
Impacting Inflammation through Mechanistic Target of Rapamycin (imTOR)R01AG084180 · NIA · UNIVERSITY OF KENTUCKY · PI Barbara Nikolajczyk · 2024 to 2026
$1.9M
NIA NIH HHS R01 AG079525NIA NIH HHS R01 AG084180NIGMS NIH HHS P20 GM156679
6 · The paper itself

Abstract

Stable isotope tracing gives direct insight into the rate of metabolic reactions occurring in cells by analyzing the incorporation of labeled carbons into metabolic pathways. Here, we present a protocol for stable isotope tracing of primary human peripheral blood mononuclear cells (PBMCs). We describe the steps for culturing and exposing PBMCs to stable isotopes U

Indexed as

Isotope LabelingLeukocytes, MononuclearCarbon IsotopesGas Chromatography-Mass SpectrometryGlucoseGlutamineHumansPrimary Cell CultureCarbon-13Carbon IsotopesGlucoseGlutamineImmunologyMetabolismMetabolomics

Identifiers

PMID41950010
PMCPMC13090647

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.