Protocol to evaluate a lineage marking system in the Drosophila testis.

Muhammed Burak Bener, Stella M DiPippo, Boris M Slepchenko, Mayu Inaba

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Muhammed Burak BenerDepartment of Cell Biology, University of Connecticut Health, Farmington, CT, USA. Electronic address: mburakbener@gmail.com.

Stella M DiPippoDepartment of Cell Biology, University of Connecticut Health, Farmington, CT, USA.

Boris M SlepchenkoDepartment of Cell Biology, University of Connecticut Health, Farmington, CT, USA; Richard D. Berlin Center for Cell Analysis and Modeling, University of Connecticut School of Medicine, Farmington, CT, USA. Electronic address: boris@uchc.edu.

Mayu InabaDepartment of Cell Biology, University of Connecticut Health, Farmington, CT, USA. Electronic address: inaba@uchc.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate interpretation of genetic lineage tracing data is often confounded by the specificity and sensitivity of promoter activity and recombination rates. Thus, reported values may misrepresent targeted event frequency. Here, we present a protocol to validate a genetic tool for marking "dedifferentiation" in Drosophila testes. We describe steps for long-term live imaging to observe reporter activation and detail procedures to estimate false-positive and false-negative rates by integrating these datasets into a mathematical model. This approach enables rigorous evaluation of system performance. For complete details on the use and execution of this protocol, please refer to Bener et al.

Indexed as

Cell LineageDrosophilaTestisAnimalsMaleCell BiologyDevelopmental biologyModel OrganismsStem CellsSystems biology

Identifiers

PMID41950009

DOI10.1016/j.xpro.2026.104479

PMCPMC13091044

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.