Evidence map›Paper›PMID 41949818›Full record

ArticleAnalytical chemistry2026

Rapid Analysis of NAD and Other Phosphorylated Metabolites in Complex Biological Samples by Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry.

Adela Pravdova, Maximilian Kleinert, John Henderson, Eleni Kafkia, David Pladevall-Morera, Caio Y Yonamine, Jonas T Treebak, Tetiana Brodiazhenko, Ilya Terenin, Jan Jakub Zylicz and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Adela PravdovaDepartment of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå 907 36, Sweden.ORCID 0000-0002-8758-1789
Maximilian KleinertDepartment of Molecular Physiology of Exercise and Nutrition, German Institute of Human Nutrition (DIfE), Potsdam-Rehbruecke, Nuthetal 14558, Germany.
John HendersonNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Copenhagen N 2200, Denmark.
Eleni KafkiaNovo Nordisk Foundation Center for Stem Cell Medicine, reNEW, University of Copenhagen, Copenhagen 2200, Denmark.
David Pladevall-MoreraNovo Nordisk Foundation Center for Stem Cell Medicine, reNEW, University of Copenhagen, Copenhagen 2200, Denmark.
Caio Y YonamineNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Copenhagen N 2200, Denmark.
Jonas T TreebakNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Copenhagen N 2200, Denmark.
Tetiana BrodiazhenkoIcosagen Cell Factory OÜ, Õssu 61713, Estonia.
Ilya TereninSciLifeLab, Lund University, Lund 221 00, Sweden.
Jan Jakub ZyliczNovo Nordisk Foundation Center for Stem Cell Medicine, reNEW, University of Copenhagen, Copenhagen 2200, Denmark.
Thomas MoritzNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Copenhagen N 2200, Denmark.
Ondrej HodekDepartment of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå 907 36, Sweden.ORCID 0000-0001-8307-9575

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nucleotides and coenzymes play critical roles in energy metabolism and cellular signaling and as building blocks of nucleic acids. This work addresses the challenges in the measurement of the phosphorylated metabolites using hydrophilic interaction liquid chromatography coupled with mass spectrometry, which facilitates the separation and detection of polar metabolites. Here, we present optimized HILIC-MS/MS methods for rapid analysis of polar metabolites including nucleotides and their derivatives in complex biological matrices, such as murine adipose, skeletal, and liver tissues, human plasma, and bacteria. The developed methodologies enable separation of key nucleotides and other phosphorylated metabolites within 6 min and cofactors such as NAD

Indexed as

NADTandem Mass SpectrometryAnimalsChromatography, LiquidHumansHydrophobic and Hydrophilic InteractionsLiquid Chromatography-Mass SpectrometryLiverMiceNADPPhosphorylationNADNADP

Identifiers

PMID41949818
PMCPMC13103929

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.