Evidence map›Paper›PMID 41946891›Full record

ArticleScientific reports2026

Genomic and biological characterization of lytic phages infecting Pseudomonas syringae associated with almond bacterial blast.

Cuong V Hoang, Jonathan Fan, Hajun Lee, Lauren Bhasin, Dominic Nguyen, Ashley Duran, Ryan Quaal, Suraj Ganiger, Luna Santiago, James E Adaskaveg and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Cuong V HoangDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Jonathan FanDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Hajun LeeDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Lauren BhasinDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Dominic NguyenDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Ashley DuranDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Ryan QuaalDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Suraj GanigerDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Luna SantiagoDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
James E AdaskavegDept. of Microbiology & Plant Pathology, University of California, Riverside, USA.
Tawanda E MaguvuDepartment of Plant Pathology, University of California, Davis, CA, USA.
Florent P TrouillasDepartment of Plant Pathology, University of California, Davis, CA, USA.
Olakunle I OlawoleDept. of Microbiology & Plant Pathology, University of California, Riverside, USA. olakunle.olawole@ucr.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pseudomonas species are Gram-negative bacterial pathogens that affect a wide range of economically important crops, including almond. Increasing resistance of Pseudomonas syringae to conventional management strategies highlights the need for alternative disease control options. In this study, we isolated and characterized three lytic bacteriophages, including vB_PsyP_Mobley, vB_PsyP_Plaza, and vB_PsyP_Mission, targeting almond-infecting Pseudomonas strains. Host-range analysis across 36 isolates revealed partially overlapping infectivity profiles, with strongest activity against phylogroup 2 (PG2) almond-associated P. syringae pv. syringae and reduced infectivity against phylogenetically distinct isolates, including PG7 P. viridiflava and other crop-associated pathovars. Efficiency-of-plating assays quantitatively supported these host-range patterns. Plaque morphology and transmission electron microscopy demonstrated icosahedral capsids with short, non-contractile tails consistent with tailed dsDNA bacteriophages of the class Caudoviricetes, with vB_PsyP_Mission producing halo-associated plaques consistent with predicted extracellular polysaccharide-modifying proteins. Genomic analyses revealed compact (~ 40 kb) genomes lacking integrases, tRNAs, lysogeny-associated genes, and known virulence or antimicrobial resistance determinants. Comparative genomics showed that vB_PsyP_Plaza and vB_PsyP_Mission likely represent novel isolates within previously described species-level groups, whereas vB_PsyP_Mobley is more genomically divergent. Collectively, these results define the phenotypic and genomic characteristics of three related but distinguishable Pseudomonas phages and provide a basis for future in planta evaluation of their potential utility against almond bacterial blast.

Indexed as

BacteriophagesGenome, ViralPlant DiseasesPrunus dulcisPseudomonas PhagesPseudomonas syringaeGenomicsHost SpecificityPhylogenyAlmond bacterial blastBacteriophage biocontrolBiofilm disruptionHost rangeLytic phagesMultiplicity of infectionPhage genomics

Identifiers

PMID41946891
PMCPMC13061930

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.