Development and validation of a crystal digital PCR assay for duck Tembusu virus detection and vaccine monitoring.

Huixin Liu, Chenchen Wang, Xiaofang Wei, Sijia Pan, Kaijun Wang

Abstract readValidation Study

In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Huixin LiuCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China; College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.

Chenchen WangCollege of Animal Science and Technology, Guangxi University Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning 530004, China.

Xiaofang WeiCollege of Animal Science and Technology, Guangxi University Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning 530004, China.

Sijia PanCollege of Animal Science and Technology, Guangxi University Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning 530004, China.

Kaijun WangCollege of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China; Hunan Provincial Key Laboratory of the Traditional Chinese Medicine Agricultural Biogenomics, Changsha Medical University, Changsha 410219, China. Electronic address: kj-wang@foxmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Duck Tembusu virus (DTMUV) threatens the duck industry and public health due to its broad host range. This study established and validated a crystal digital PCR (cdPCR) assay for molecular detection of DTMUV, comparing it with quantitative real-time PCR (qPCR). A recombinant plasmid standard (pDTMUV, targeting the DTMUV E gene) served as a reference. Optimized at 58°C annealing temperature with optimal primer/probe concentrations, cdPCR showed high specificity (only DTMUV positive) and precision (low intra-/inter-assay CVs), with a limit of detection (LOD) of 0.19 copies/μL-100-fold more sensitive than qPCR (2.68×10

Indexed as

DucksFlavivirusFlavivirus InfectionsPolymerase Chain ReactionPoultry DiseasesReal-Time Polymerase Chain ReactionViral VaccinesAnimalsSensitivity and SpecificityViral VaccinesClinical samplesCrystal digital PCR (cdPCR)Duck Tembusu virus (DTMUV)Molecular detectionVaccine monitoring

Identifiers

PMID41946073

DOI10.1016/j.psj.2026.106832

PMCPMC13090510

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.