Evidence map›Paper›PMID 41945114›Full record

ArticleArchives of microbiology2026

Transcriptional regulator Rv2250c affects isoniazid resistance by down-regulating the expression of efflux pump Rv3728 in Mycobacterium tuberculosis.

Hui Wang, Yingying Cui, Guanghui Dang, Jiajun Zhang, Yiyi Tang, Tingting Feng, Xinxin Zang, Ningning Song, Siguo Liu

Abstract read
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In one paragraph

Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hui Wang *State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Yingying Cui *State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Guanghui DangState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Jiajun ZhangState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Yiyi TangState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Tingting FengState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Xinxin ZangState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Ningning SongBioengineering Department, School of Life Science and Technology, Shandong Second Medical University, Weifang, 261000, China.
Siguo LiuState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. liusiguo@caas.cn.

Funding

Central Public-interest Scientific Institution Basal Research Fund 1610302024002National Key Research and Development Program of China 2021YFD1800403National Natural Science Foundation of China 32002256National Natural Science Foundation of China 32273005
6 · The paper itself

Abstract

Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis, is currently resistant to antibiotics and has undergone transcriptional adaptation to them, yet only a few transcription factors regulating mycobacterial drug resistance have been identified. In this study, the transcriptional regulatory protein Rv2250c of the TetR family was found to be a regulatory molecule of INH. Compared with wild-type strains, strains lacking the rv2250c gene showed higher minimum inhibitory concentrations (MICs) and greater intracellular survival in macrophages under INH stress. Furthermore, the INH competition with Rv2250c weakens its interaction with the efflux pump gene rv3728, thereby reducing its negative regulatory effect. This enhances the function of the MTB cell wall efflux pump, reducing the accumulation of ethidium bromide (EtBr) within the bacteria. In summary, we found that Rv2250c can regulate INH susceptibility by modulating the expression of efflux pump-encoding genes.

Indexed as

Antitubercular AgentsBacterial ProteinsDrug Resistance, BacterialIsoniazidMembrane Transport ProteinsMycobacterium tuberculosisTranscription FactorsDown-RegulationGene Expression Regulation, BacterialMacrophagesMicrobial Sensitivity TestsAntitubercular AgentsBacterial ProteinsIsoniazidMembrane Transport ProteinsTranscription FactorsEfflux pump Rv3728IsoniazidMycobacterium tuberculosisTranscriptional regulatory protein Rv2250c

Identifiers

PMID41945114

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.