Evidence map›Paper›PMID 41944605›Full record

ArticlemBio2026

Mutation of lysine 396 of the fusion protein reduces its binding affinity with integrin αVβ1 and leads to attenuation of avian metapneumovirus subtype B.

Lingzhai Meng, Yuntong Chen, Mengmeng Yu, Yongzhen Liu, Suyan Wang, Xiaoxiao Xue, Wenrui Fan, Ying Wang, Tao Zhang, Peidong Guo and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Lingzhai MengAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.ORCID 0009-0003-4537-8701
Yuntong ChenAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Mengmeng YuAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Yongzhen LiuAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Suyan WangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Xiaoxiao XueAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Wenrui FanAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Ying WangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Tao ZhangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Peidong GuoAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Ru GuoAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Mingxue HuAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Xiaole QiAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Yanping ZhangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Yulu DuanAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Hongyu CuiAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.
Yulong GaoAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, China.ORCID 0000-0002-0448-8467

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Avian metapneumovirus subtype B (aMPV/B) infections significantly affect the global poultry industry. However, the virulence determinants and attenuation mechanism remain unknown. Here, a series of chimeric and mutant viruses was constructed, and their pathogenicity was evaluated in a specific-pathogen-free (SPF) chicken model. First, substitutions in different genes (N, P, F, SH, G, and L) and amino acid sites (323rd, 396th, and 522nd residues in F protein) in virulent (LN16-V) and attenuated (LN16-A) viruses revealed that the residue 396th in F protein is closely related to replication ability

Indexed as

LysineMetapneumovirusParamyxoviridae InfectionsViral Fusion ProteinsAnimalsCell LineChickensMutationPoultry DiseasesProtein BindingSpecific Pathogen-Free OrganismsVirulenceVirus ReplicationLysineViral Fusion Proteinsattenuation mechanismavian metapneumovirus subtype BbindingvirulenceαVβ1 integrin

Identifiers

PMID41944605
PMCPMC13170324

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.