Evidence map›Paper›PMID 41943926›Full record

ArticleBiology open2026

StrIPETrack: a real-time, ROI-flexible tracking platform for high-throughput zebrafish behavior.

Camden E Cummings, Brandon L Bastien, Jaqueline A Martinez, Jinyue Luo, Summer B Thyme

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

  • Update of
    2026
5 · Who and what money

Authors and funding

5 authors.

Camden E CummingsDepartment of Biochemistry and Molecular Biotechnology, The University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Brandon L BastienDepartment of Biochemistry and Molecular Biotechnology, The University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Jaqueline A MartinezDepartment of Biochemistry and Molecular Biotechnology, The University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Jinyue LuoDepartment of Biochemistry and Molecular Biotechnology, The University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Summer B ThymeDepartment of Biochemistry and Molecular Biotechnology, The University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0003-3593-4148

Funding

Large-scale Genetic Investigation and Drug Discovery to Counteract Down Syndrome Neural DeficitsR01HD115159 · NICHD · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Summer B Thyme · 2024 to 2026
$2.5M
Defining the Chemical Perturbome of Neural Development and ActivityDP2NS132107 · NINDS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI THYME, SUMMER B · 2022 to 2025
$2.3M
National Institute of Child Health and Human Development HD115159NICHD NIH HHS R01 HD115159NIH HHS DP2NS132107NIH HHS HD115159NIH Office of the Director NS132107NINDS NIH HHS DP2 NS132107Simons FoundationUMass Chan Medical School
6 · The paper itself

Abstract

Quantitative phenotyping is essential to studies of animal behavior, enabling systematic analysis of variation arising from natural diversity or experimental manipulation. High-throughput behavioral assays that can simultaneously test multiple animals support sufficiently powered studies of behavioral variation, but accurate tracking of each animal is critical. Furthermore, behavioral tasks and experimental arenas span a wide range of complexity, from the reaction of a single larval zebrafish to an acoustic stimulus to associative conditioning in cue-rich environments. Here, we developed and validated StrIPETrack (Structural similarity-based Image Processing for Estimation and Tracking), a Python-based, modular animal tracking software designed for flexible region-of-interest definitions and extensibility across assays. We show that StrIPETrack measures activity comparably to our previous LabVIEW-based zebrafish tracking software and detects similar behavioral differences between wild-type clutches. In addition, StrIPETrack accurately captures behavior in a complex arena: the Y-maze. Our approach for analyzing Y-maze navigation yields an expanded set of metrics beyond turn count and direction, revealing more subtle behavioral variation. Overall, this versatile software can be applied to monitor the activity of multiple animals in parallel in both simple, high-throughput and more complex assays, and it can be readily adapted to new paradigms.

Indexed as

Behavior, AnimalHigh-Throughput Screening AssaysImage Processing, Computer-AssistedSoftwareZebrafishAnimalsHigh-throughput behaviorOpen-sourceSpatial navigationWorking memoryY-mazeZebrafish

Identifiers

PMID41943926
PMCPMC13148472

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.