Evidence map›Paper›PMID 41943637›Full record

ArticleDisease models & mechanisms2026

A zebrafish luminescent biosensor for kidney tubulopathy, metal toxicity and drug screening.

Han Lai, Monica Goldade, Svenja Aline Keller, Isabelle Worms, Alessandro Luciani, Stephan C F Neuhauss, Vera I Slaveykova, Olivier Devuyst, Zhiyong Chen

Abstract read
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In one paragraph

Article in Disease models & mechanisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Han LaiDepartment of Physiology, University of Zurich, 8057 Zurich, Switzerland.
Monica GoldadeDepartment of Physiology, University of Zurich, 8057 Zurich, Switzerland.
Svenja Aline KellerDepartment of Physiology, University of Zurich, 8057 Zurich, Switzerland.
Isabelle WormsDepartment F.-A. Forel for Environmental and Aquatic Sciences, School of Earth and Environmental Sciences, Faculty of Science, University of Geneva, CH-1211 Geneva 4, Switzerland.
Alessandro LucianiDepartment of Physiology, University of Zurich, 8057 Zurich, Switzerland.
Stephan C F NeuhaussDepartment of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland.
Vera I SlaveykovaDepartment F.-A. Forel for Environmental and Aquatic Sciences, School of Earth and Environmental Sciences, Faculty of Science, University of Geneva, CH-1211 Geneva 4, Switzerland.
Olivier DevuystDepartment of Physiology, University of Zurich, 8057 Zurich, Switzerland.
Zhiyong ChenDepartment of Physiology, University of Zurich, 8057 Zurich, Switzerland.ORCID 0000-0003-0203-2930

Funding

Cystinosis Research Foundation CRFS-2017-007Cystinosis Research Foundation CRFS-2020-005Cystinosis Research Foundation CRFS-2023-003European Reference Network for Rare Kidney DiseasesNational Center of Competence in Research-Kidney Control of HomeostasisNational Natural Science Foundation of China 82100752Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 310030_215715Third Framework Programme 2017-2021Universitat Zurich 4141100Universität Zürich 4141100
6 · The paper itself

Abstract

An efficient endolysosomal pathway is crucial to mediate the reabsorption and processing of ultrafiltered solutes including low-molecular-weight (LMW) proteins by epithelial cells lining the proximal tubule (PT) of the kidney. The zebrafish pronephros is used as a model system for congenital or acquired disorders that impair endolysosomal processing in PT cells, causing inappropriate loss of solutes and LMW proteins in urine. Here, we describe a new reporter ½vdbp-NanoLuc zebrafish line, in which vitamin D-binding protein is coupled to NanoLuc luciferase for detection of PT dysfunction and LMW proteinuria. We demonstrate the reliability and value of the ½vdbp-NanoLuc biosensor in fish models of monogenic endolysosomal diseases, gentamicin and cisplatin-induced nephrotoxicity, and metal contamination. This novel reporter system yields mechanistic insights into cadmium- and copper-induced PT dysfunction and provides a platform for drug screening.

Indexed as

Biosensing TechniquesKidney DiseasesKidney TubulesMetalsZebrafishAnimalsCisplatinCopperDrug Evaluation, PreclinicalEndocytosisGenes, ReporterGentamicinsKidney Tubules, ProximalLuciferasesLysosomesProteinuriaCisplatinCopperGentamicinsLuciferasesMetalsLow-molecular-weight proteinuriaLysosomeNanoLuc luciferaseProximal tubuleReceptor-mediated endocytosis

Identifiers

PMID41943637
PMCPMC13225708

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.