ArticleScientific reports2026
Effect of nanoliposomal gamma-oryzanol on cryosurvival, oxidative balance, and fertility of frozen-thawed ram spermatozoa.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study was to examine how gamma-oryzanol (GO) and gamma-oryzanol nanoparticles (GO-N) affected the quality of ram sperm after freeze-thaw treatment. Epididymal sperm from Shall rams collected from a slaughterhouse were diluted and divided into seven groups: a control group (0 µM), three concentrations of GO (50, 100, and 200 µM), and three concentrations of GO-N (50, 100, and 200 µM). The results demonstrated that treatment with 100 µM GO-N significantly improved the total motility (70.45%), progressive motility (60.21%), and velocity parameters (VCL, VSL, and VAP) compared to the other groups (p < 0.05). Plasma membrane and acrosomal integrity increased to 75.76% and 77.35%, respectively, while the percentage of morphological abnormalities decreased to 16.67% (p < 0.05). In the same group, flow cytometry revealed a reduction in DNA fragmentation to 17.22%, an increase in mitochondrial membrane potential to 66.11%, a decrease in H₂O₂ concentration to 13.29%, and the highest sperm viability (76.76%). MDA levels dropped to 3.42 nmol/mL, while SOD and GPx activities increased to 138.16 and 74.69 U/mg protein, respectively. Fertility assessments also indicated that the use of 100 µM GO-N in the extender significantly improved the pregnancy rate, lambing rate, and prolificacy compared with the other treatments (p < 0.05). In conclusion, the addition of 100 µM GO-N to the cryopreservation extender appears to be an effective strategy for enhancing the quality and functionality of thawed ram sperm, and may be valuable in breeding programs, reproductive biotechnology, and assisted reproductive technologies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.