Evidence map›Paper›PMID 41942464›Full record

ArticleNature communications2026

Identification of stem cell marker-positive subpopulations in the vocal fold of the larynx through transcriptomic analyses.

Keiichi Tamura, Hiroe Ohnishi, Koki Hasebe, Shintaro Fujimura, Tatsuya Katsuno, Zhaonan Zou, Shinya Oki, Yasuyuki Ohkawa, Satoshi Morita, Koichi Omori

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Keiichi Tamura *Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.ORCID http://orcid.org/0009-0009-7134-2204
Hiroe Ohnishi *Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.ORCID http://orcid.org/0000-0003-3437-680X
Koki HasebeDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.ORCID http://orcid.org/0000-0003-2555-0511
Shintaro FujimuraDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.ORCID http://orcid.org/0000-0002-6564-5876
Tatsuya KatsunoCenter of Anatomical studies, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Zhaonan ZouInstitute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan.ORCID http://orcid.org/0000-0002-1075-4936
Shinya OkiInstitute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan.ORCID http://orcid.org/0000-0002-4767-3259
Yasuyuki OhkawaDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.ORCID http://orcid.org/0000-0001-6440-9954
Satoshi MoritaDepartment of Biomedical Statistics and Bioinformatics, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Koichi OmoriDepartment of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan. omori@ent.kuhp.kyoto-u.ac.jp.ORCID http://orcid.org/0000-0003-3404-5461

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Information on the maintenance of tissue homeostasis is important for developing effective therapeutic methods. However, reports on the cellular composition and tissue stem cells of the larynx are scarce. Therefore, we analyzed mouse laryngeal mucosa using single-cell RNA- sequencing and spatial transcriptomics by photo-isolation chemistry, and we also generated laryngeal organoids as an in vitro model. Consequently, we found a SOX9-positive basal cell subpopulation and a Lgr5-positive cell subpopulation in the mouse vocal fold, and obtained three types of epithelial organoids from laryngeal epithelium. We also confirmed the differences in pseudostratified ciliated columnar epithelium characters between the supraglottis and subglottis of the mouse larynx. These findings provide valuable insights and tools for future research in laryngology and stem cell biology.

Indexed as

Stem CellsVocal CordsAnimalsBiomarkersGene Expression ProfilingLaryngeal MucosaMaleMiceMice, Inbred C57BLOrganoidsReceptors, G-Protein-CoupledSingle-Cell Gene Expression AnalysisSOX9 Transcription FactorSpatial TranscriptomicsTranscriptomeBiomarkersLgr5 protein, mouseReceptors, G-Protein-CoupledSox9 protein, mouseSOX9 Transcription Factor

Identifiers

PMID41942464
PMCPMC13233822

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.