Evidence map›Paper›PMID 41941272›Full record

ArticleACS sensors2026

Peptide Aptamer-Enabled Nanoplasmonic Digital Immunoassay for Ultrasensitive Cytokine Sensing in Early Inflammation and Immune Modulation.

Shuai Wu, Lang Zhou, Xuejia Kang, Gangtong Huang, Yoolim Cha, Jialiang Shen, Chuanyu Wang, Kimberly H Lackey, Dong-Joo Kim, Siyuan Dai and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS sensors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Shuai WuMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.
Lang ZhouMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.ORCID 0000-0002-4316-1192
Xuejia KangMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.ORCID 0000-0003-4336-2098
Gangtong HuangDepartment of Physics and Astronomy, Clemson University, Clemson, South Carolina 29634, USA.ORCID 0000-0001-8631-7014
Yoolim ChaMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.ORCID 0000-0002-9955-4139
Jialiang ShenMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.
Chuanyu WangMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.
Kimberly H LackeyDepartment of Biological Sciences, The University of Alabama, Tuscaloosa, Alabama 35487, USA.
Dong-Joo KimMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.ORCID 0000-0001-5013-4005
Siyuan DaiMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.
Feng DingDepartment of Physics and Astronomy, Clemson University, Clemson, South Carolina 29634, USA.ORCID 0000-0003-1850-6336
Pengyu ChenMaterials Engineering, Department of Mechanical Engineering, Auburn University, Auburn, Alabama 36849, USA.ORCID 0000-0003-3380-872X

Funding

Purchase of a modularized confocal microscopeR35GM133795 · NIGMS · AUBURN UNIVERSITY AT AUBURN · PI Pengyu Chen · 2019 to 2026
$3.3M
Inhibition of Human Islet Amyloid Polypeptide AggregationR35GM145409 · NIGMS · CLEMSON UNIVERSITY · PI Feng Ding · 2022 to 2026
$2.0M
NIGMS NIH HHS R35 GM133795NIGMS NIH HHS R35 GM145409
6 · The paper itself

Abstract

Early and precise detection of cytokine release is essential for mitigating cytokine release syndrome, a life-threatening complication of CAR T-cell therapy. However, existing immunoassays fall short in sensitivity, dynamic range, and temporal resolution, limiting their ability to capture the subtle, transient cytokine fluctuations that mark the earliest phase of immune activation. To address this unmet need, we developed an ultrasensitive nanoplasmonic digital immunoassay that integrates rationally engineered antibody-derived peptide aptamers (ADPAs), plasmonic gold nanospheres for digital dark-field imaging, and convolutional neural network signal quantification. This platform enables ultrasensitive cytokine detection down to tens of fg/mL, with quantitative coverage across the tested concentration range spanning approximately six orders of magnitude using only microliter-scale sample volumes. We applied this technology to a physiologically relevant

Indexed as

Aptamers, PeptideBiosensing TechniquesCytokinesInflammationNanotechnologyGoldHumansImmunoassayMetal NanoparticlesAptamers, PeptideCytokinesGoldantibody-derived peptide aptamer (ADPA)cytokine release syndromeearly immune activation monitoringnanoplasmonic Immunoassayultrasensitive cytokine detection

Identifiers

PMID41941272
PMCPMC13156957

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.