Evidence map›Paper›PMID 41940584›Full record

ArticlePlant, cell & environment2026

The AcWRKY40-AcICS1 Module Regulates Kiwifruit Resistance to Pseudomonas syringae pv. actinidiae via Salicylic Acid Synthesis.

Xiaoya Wang, Yuanyuan Liu, Jilong Cheng, Shuangrong Li, Gaopin Wu, Xueren Yin, Lili Huang, Gongyou Chen, Hanrao Zhang, Yue Huang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant, cell & environment, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xiaoya WangAnhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui, China.
Yuanyuan LiuAnhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui, China.
Jilong ChengAnhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui, China.
Shuangrong LiAnhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui, China.
Gaopin WuAnhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui, China.
Xueren YinAnhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui, China.
Lili HuangState Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A & F University, Yangling, Shaanxi, China.ORCID https://orcid.org/0000-0002-7085-7646
Gongyou ChenState Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Hanrao ZhangKey Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, Yunnan, China.ORCID https://orcid.org/0000-0001-7440-4348
Yue HuangAnhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui, China.ORCID https://orcid.org/0000-0002-6572-2341
Pu LiuAnhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui, China.ORCID https://orcid.org/0000-0002-2624-7998

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is one of the most destructive diseases affecting kiwifruit production worldwide. Salicylic acid (SA) plays a pivotal role in plant immunity, with its levels and signaling tightly regulated to maintain a balanced immune response. In plants, SA is biosynthesized via two distinct pathways: the isochorismate synthase (ICS) pathway and the phenylalanine ammonia-lyase (PAL) pathway. However, their roles in kiwifruit remain unclear. Here, we demonstrate that AcICS1 positively regulates kiwifruit resistance to Psa by promoting SA biosynthesis. Overexpression of AcICS1 significantly enhanced disease resistance, whereas silencing AcICS1 compromised immunity. Through dual-luciferase assays, AcWRKY40 was identified as a candidate regulator of AcICS1. Its direct binding to the W-box motif in the AcICS1 promoter was further confirmed by electrophoretic mobility shift assay (EMSA) and yeast one-hybrid (Y1H) assays. Functional analyses revealed that AcWRKY40 acts as a negative regulator of kiwifruit resistance to Psa: overexpression of AcWRKY40 repressed AcICS1 expression, inhibited SA accumulation, and increased disease susceptibility, whereas silencing AcWRKY40 alleviated these effects. Collectively, these findings identify AcWRKY40 as a transcriptional repressor of AcICS1, establish the AcWRKY40-AcICS1 regulatory module in kiwifruit, and provide new insights into SA-mediated resistance to bacterial canker.

Indexed as

ActinidiaDisease ResistancePlant DiseasesPlant ProteinsPseudomonas syringaeSalicylic AcidTranscription FactorsGene Expression Regulation, PlantPromoter Regions, GeneticPlant ProteinsSalicylic AcidTranscription Factorsisochorismate synthasekiwifruitPseudomonas syringae pv. actinidiaesalicylic acidWRKY

Identifiers

PMID41940584
PMCPMC13353690

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.