Evidence map›Paper›PMID 41938127›Full record

ArticleSynthetic and systems biotechnology2026

Semi-rational engineering of terminal deoxynucleotidyl transferase for high-efficiency enzymatic

Chengjie Zhang, Zhicong He, Zhengquan Gao, Chunxiao Meng, Wei Peng, Lei Du, Shengying Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Synthetic and systems biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Engineering a Virus-Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chengjie ZhangState Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, China.
Zhicong HeSchool of Water and Environment, Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Key Laboratory of Ecohydrology and Water Security in Arid and Semi-Arid Regions of Ministry of Water Resources, Chang'an University, Xi'an, 710054, China.
Zhengquan GaoSchool of Pharmacy, Binzhou Medical University, Yantai, 264003, China.
Chunxiao MengSchool of Pharmacy, Binzhou Medical University, Yantai, 264003, China.
Wei PengState Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, China.
Lei DuState Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, China.
Shengying LiState Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The 21st century has witnessed rapid advancements in synthetic biology, with DNA synthesis emerging as a foundational technology. Conventional phosphoramidite-based methods face significant limitations, including short DNA elongation lengths (<300 nt), hazardous chemical waste, and low stepwise incorporation efficiency. Enzymatic DNA synthesis using terminal deoxynucleotidyl transferase (TdT) offers a promising alternative, enabling kilobase-scale assembly with greater efficiency and minimal environmental impact. Here, we identified

Indexed as

3′–ONH2–dNTPsDNA synthesisEnzyme engineeringTerminal deoxynucleotidyl transferase

Identifiers

PMID41938127
PMCPMC13050093

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.