Evidence map›Paper›PMID 41935790›Full record

ArticleVirus research2026

The influenza B virus candidate vaccine expressing H3 hemagglutinin developed in suspension MDCK cells confers protection against lethal H3N2 avian influenza in BALB/c mice.

Yushu Wu, Weiyang Sun, Yeting Xia, Yue Feng, Menglin Zhao, Tiecheng Wang, Xianzhu Xia, Fang Yan, Yuwei Gao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yushu WuChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China. Electronic address: wuyushu0522@163.com.
Weiyang SunChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China. Electronic address: 891209604@qq.com.
Yeting XiaCollege of life sciences, Henan University, Kaifeng 475004, China. Electronic address: 1095140503@qq.com.
Yue FengChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China; College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China. Electronic address: fengyuesx12@outlook.com.
Menglin ZhaoHebei Animal Disease Prevention and Control Center, Shijiazhuang, Hebei 050035, China. Electronic address: 182xdyh@sina.cn.
Tiecheng WangChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China. Electronic address: wgcha@163.com.
Xianzhu XiaChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China. Electronic address: xiaxzh@cae.cn.
Fang YanCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China. Electronic address: yanfang6615@163.com.
Yuwei GaoChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China. Electronic address: dawei1105@foxmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Influenza viruses cause annual seasonal epidemics and occasional pandemics. Vaccination is most effective at preventing influenza virus infections. Suspensions of the Madin-Darby canine kidney (MDCKs) cell line are used to manufacture cell-based influenza vaccines. In this study, we tested and optimized a range of culture conditions for a chimeric recombinant influenza virus rA/B-H3 in MDCK suspension cells, revealing that the optimal inoculation conditions were multiplicity of infection of 0.001, cell concentration of infection of 4.0 × 10

Indexed as

Hemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H3N2 SubtypeInfluenza B virusInfluenza VaccinesOrthomyxoviridae InfectionsAnimalsAntibodies, NeutralizingAntibodies, ViralDogsFemaleHemagglutination Inhibition TestsImmunoglobulin AImmunoglobulin GInterferon-gammaMadin Darby Canine Kidney CellsMiceAntibodies, NeutralizingAntibodies, ViralHemagglutinin Glycoproteins, Influenza VirusImmunoglobulin AImmunoglobulin GInfluenza VaccinesInterferon-gammaVaccines, SyntheticBALB/c miceChimeric vaccineImmunizationMDCK suspension cellProcess optimization

Identifiers

PMID41935790
PMCPMC13090522

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.