Evidence map›Paper›PMID 41934607›Full record

ArticleJournal of veterinary internal medicine2026

Seroprevalence of Sarcocystis neurona and Neospora hughesi in healthy horses from the province of Alberta, Canada.

Erika Sjolin, Luiza S Zakia, Angelica Galezowski, Ashley E Whitehead

Abstract read
In one paragraph

Article in Journal of veterinary internal medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Erika SjolinDepartment of Clinical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Canada.ORCID 0009-0004-4354-5761
Luiza S ZakiaDepartment of Clinical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Canada.ORCID 0000-0003-1022-2507
Angelica GalezowskiFaculty of Veterinary Medicine, University of Calgary, Calgary, Canada.ORCID 0000-0002-4863-8295
Ashley E WhiteheadFaculty of Veterinary Medicine, University of Calgary, Calgary, Canada.ORCID 0000-0002-3165-6809

Funding

Equine Diagnostic SolutionsFaculty start-up fundsUniversity of Calgary Faculty of Veterinary Medicine Equine Health FundsUniversity of Calgary Faculty of Veterinary Medicine Internship Research Fund
6 · The paper itself

Abstract

backgroundLiterature on the seroprevalence of antibodies to the causative agents of equine protozoal myeloencephalitis (EPM) in Canada is limited. HYPOTHESIS/

objectivesTo determine the prevalence of serum antibodies against Sarcocystis neurona and Neospora hughesi in apparently healthy horses without signs of neurologic disease which have always resided in Alberta, Canada, using commercially available immunodiagnostic tests. ANIMALS: Samples from 116 healthy horses were obtained from a biobank containing horse serum collected across Alberta in 2021 and 2022. Samples were selected based on owner survey responses, and only horses that had always resided in Alberta were included in the study.

methodsThis cross-sectional study analyzed serum samples for anti-S neurona (SarcoFluor and rSnSAG2/4/3 ELISA) and N hughesi (NeoFluor and NhSAG1 ELISA) antibodies. A subset of 30 samples underwent additional testing by S neurona western blot.

resultsFor anti-S neurona antibodies, 26/116 horses were positive on indirect fluorescent antibody test (IFAT), 4/116 on ELISA, and 5/30 on western blot; however, none of the horses were positive on all 3 of the tests. For anti-N hughesi antibodies, 18/116 were positive on IFAT and 2/116 on ELISA, with no horses positive on both tests. CONCLUSIONS AND CLINICAL IMPORTANCE: These findings suggest possible exposure of Alberta horses to S neurona and N hughesi. However, the commercially available tests had low sensitivities and poor agreement in this study group of apparently healthy horses.

Indexed as

CoccidiosisHorse DiseasesNeosporaSarcocystisSarcocystosisAlbertaAnimalsAntibodies, ProtozoanBlotting, WesternCross-Sectional StudiesEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody Technique, IndirectHorsesMaleSeroepidemiologic StudiesAntibodies, ProtozoanELISAequineequine protozoal myeloencephalitisIFATwestern blot

Identifiers

PMID41934607
PMCPMC13050048

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.